Literature DB >> 3993925

Simultaneous extraction and reverse-phase high-performance liquid chromatographic determination of adenine and pyridine nucleotides in human red blood cells.

V Stocchi, L Cucchiarini, M Magnani, L Chiarantini, P Palma, G Crescentini.   

Abstract

A simple and rapid method for the determination of ATP, ADP, AMP, NADP+, NAD+, NADPH, and NADH in human erythrocytes is described. A single-step extraction procedure employing alkaline medium and CF 50A Amicon ultrafiltration membranes allows a simultaneous and total recovery of the compounds of interest. Analysis is performed by reverse-phase high-performance liquid chromatography on a 5-micron Supelcosil LC-18 column and uv detection. Extraction and analysis require about 30 min. Levels of adenine and pyridine nucleotides in normal adults are also presented.

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Year:  1985        PMID： 3993925     DOI： 10.1016/0003-2697(85)90405-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  58 in total

1.  Mitochondrial biogenesis by NO yields functionally active mitochondria in mammals.

Authors:  Enzo Nisoli; Sestina Falcone; Cristina Tonello; Valeria Cozzi; Letizia Palomba; Mara Fiorani; Addolorata Pisconti; Silvia Brunelli; Annalisa Cardile; Maura Francolini; Orazio Cantoni; Michele O Carruba; Salvador Moncada; Emilio Clementi
Journal:  Proc Natl Acad Sci U S A       Date:  2004-11-15       Impact factor: 11.205

2.  Glucose metabolism in fibroblasts from patients with erythrocyte hexokinase deficiency.

Authors:  M Magnani; L Chiarantini; V Stocchi; M Dachà; G Fornaini
Journal:  J Inherit Metab Dis       Date:  1986       Impact factor: 4.982

3.  A LC-MS/MS Method for Quantifying Adenosine, Guanosine and Inosine Nucleotides in Human Cells.

Authors:  Leah C Jimmerson; Lane R Bushman; Michelle L Ray; Peter L Anderson; Jennifer J Kiser
Journal:  Pharm Res       Date:  2016-09-15       Impact factor: 4.200

4.  Metabolite patterns related to exhaustion, recovery and transformation of chronically stimulated rabbit fast-twitch muscle.

Authors:  H J Green; S Düsterhöft; L Dux; D Pette
Journal:  Pflugers Arch       Date:  1992-03       Impact factor: 3.657

5.  Activation of IGF-1 and insulin signaling pathways ameliorate mitochondrial function and energy metabolism in Huntington's Disease human lymphoblasts.

Authors:  Luana Naia; I Luísa Ferreira; Teresa Cunha-Oliveira; Ana I Duarte; Márcio Ribeiro; Tatiana R Rosenstock; Mário N Laço; Maria J Ribeiro; Catarina R Oliveira; Frédéric Saudou; Sandrine Humbert; A Cristina Rego
Journal:  Mol Neurobiol       Date:  2014-05-20       Impact factor: 5.590

6.  Metabolic Effects of Dietary Glycerol Supplementation in Muscle and Liver of European Seabass and Rainbow Trout by 1H NMR Metabolomics.

Authors:  Mariana Palma; Ludgero C Tavares; João Rito; Luís F Henriques; João G Silva; Rodrigo Ozório; Miguel A Pardal; Leonardo J Magnoni; Ivan Viegas
Journal:  Metabolites       Date:  2019-09-27

7.  Adenosine diphosphate involvement in THP-1 maturation triggered by the contact allergen 1-fluoro-2,4-dinitrobenzene.

Authors:  J D Martins; A Silva; I Ferreira; M Gonçalo; J B A Custódio; M C Lopes; M R M Domingues; B M Neves; M T Cruz
Journal:  Toxicol Res (Camb)       Date:  2016-08-04       Impact factor: 3.524

8.  The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans.

Authors:  Javier Apfeld; Greg O'Connor; Tom McDonagh; Peter S DiStefano; Rory Curtis
Journal:  Genes Dev       Date:  2004-12-01       Impact factor: 11.361

9.  Metabolic alterations in hepatocytes promoted by the herbicides paraquat, dinoseb and 2,4-D.

Authors:  C M Palmeira; A J Moreno; V M Madeira
Journal:  Arch Toxicol       Date:  1994       Impact factor: 5.153

10.  Adenosine nucleotide biosynthesis and AMPK regulate adult life span and mediate the longevity benefit of caloric restriction in flies.

Authors:  Drew Stenesen; Jae Myoung Suh; Jin Seo; Kweon Yu; Kyu-Sun Lee; Jong-Seok Kim; Kyung-Jin Min; Jonathan M Graff
Journal:  Cell Metab       Date:  2013-01-08       Impact factor: 27.287
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