Literature DB >> 3990528

Lipid composition of different areas of murine brain: effects of lipid extraction procedures.

S L Katyal, L Barilaro, I Hanin.   

Abstract

The effects of various chemical extraction procedures on the determination of lipid composition of rat and mouse brain have been investigated. Tissue extractions with formic acid/acetone or perchloric acid both resulted in significant losses of total phospholipids and cholesterol. Perchloric acid extraction also degraded, almost quantitatively, ethanolamine plasmalogens to lysophosphatidylethanolamine. Our findings have thus demonstrated that conventional procedures used for extraction of brain tissue for analysis of choline and acetylcholine content cannot also be used for concurrent/simultaneous extraction of phospholipids and cholesterol from the same tissue.

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Year:  1985        PMID： 3990528     DOI： 10.1007/bf02534255

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  6 in total

1.  Analysis of microquantities of choline and its esters utilizing gas chromatography-chemical ionization mass spectrometry.

Authors:  I Hanin; R F Skinner
Journal:  Anal Biochem       Date:  1975-06       Impact factor: 3.365

2.  A rapid method of total lipid extraction and purification.

Authors:  E G BLIGH; W J DYER
Journal:  Can J Biochem Physiol       Date:  1959-08

3.  A rapid and specific ultramicro method for total serum cholesterol.

Authors:  R E BOWMAN; R C WOLF
Journal:  Clin Chem       Date:  1962 May-Jun       Impact factor: 8.327

4.  Quantitation of phosphatidyl N-methyl and N,N-dimethyl aminoethanol in liver and lung of N-methylaminoethanol fed rats.

Authors:  S L Katyal; B Lombardi
Journal:  Lipids       Date:  1974-02       Impact factor: 1.880

5.  The phospholipids of various sheep organs, rat liver and of their subcellular fractions.

Authors:  G S Getz; W Bartley; D Lurie; B M Notton
Journal:  Biochim Biophys Acta       Date:  1968-03-04

6.  The alk-1-enyl group content of mammalian myelin phosphoglycerides by quantitative two-dimensional thin-layer chromatography.

Authors:  L A Horrocks
Journal:  J Lipid Res       Date:  1968-07       Impact factor: 5.922
  6 in total
  5 in total

1.  C-terminal domain of mammalian complexin-1 localizes to highly curved membranes.

Authors:  Jihong Gong; Ying Lai; Xiaohong Li; Mengxian Wang; Jeremy Leitz; Yachong Hu; Yunxiang Zhang; Ucheor B Choi; Daniel Cipriano; Richard A Pfuetzner; Thomas C Südhof; Xiaofei Yang; Axel T Brunger; Jiajie Diao
Journal:  Proc Natl Acad Sci U S A       Date:  2016-11-07       Impact factor: 11.205

2.  N-terminal domain of complexin independently activates calcium-triggered fusion.

Authors:  Ying Lai; Ucheor B Choi; Yunxiang Zhang; Minglei Zhao; Richard A Pfuetzner; Austin L Wang; Jiajie Diao; Axel T Brunger
Journal:  Proc Natl Acad Sci U S A       Date:  2016-07-21       Impact factor: 11.205

3.  Response to Comments on "Magnetic Resonance Spectroscopy Identifies Neural Progenitor Cells in the Live Human Brain".

Authors:  Petar M Djurić; Helena Benveniste; Mark E Wagshul; Fritz Henn; Grigori Enikolopov; Mirjana Maletić-Savatić
Journal:  Science       Date:  2008-08-01       Impact factor: 47.728

4.  Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease.

Authors:  Cécile Bascoul-Colombo; Irina A Guschina; Benjamin H Maskrey; Mark Good; Valerie B O'Donnell; John L Harwood
Journal:  Biochim Biophys Acta       Date:  2016-03-09

5.  Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18.

Authors:  Ying Lai; Ucheor B Choi; Jeremy Leitz; Hong Jun Rhee; Choongku Lee; Bekir Altas; Minglei Zhao; Richard A Pfuetzner; Austin L Wang; Nils Brose; JeongSeop Rhee; Axel T Brunger
Journal:  Neuron       Date:  2017-08-02       Impact factor: 17.173
  5 in total

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