gamma-Amino butyric-N-acid sensitivity of mouse and human oocytes.

gamma-Aminobutyric acid (GABA)-sensitivity was studied in mouse and human oocytes using electrophysiological techniques. Isolated mouse oocytes at the germinal vesicle (GV) or metaphase II stage, and human oocytes at the GV stage or following resumption of meiosis in culture, were sensitive to GABA. The transmitter usually hyperpolarized the membrane, with a concomitant decrease followed by an increase in membrane conductance, at threshold concentrations as low as 10(-10) M. GABA response was not evoked in Cl-free medium. Bicuculline (10(-5)-10(-6) M) reversibly blocked GABA (10(-9)-10(-4) M) responses. In contrast mouse fertilized eggs were insensitive to GABA at concentrations of 10(-5) M or lower. A possible biological role of the neurotransmitter GABA is discussed.