An H1-like protein from the sperm chromatin of Mytilus galloprovincialis.

We have isolated and purified a sperm-specific protein (S3) from the mussel M. galloprovincialis. Antibodies against S3 were raised in rabbits and used for its immunological comparison to somatic histones. The results showed that S3 did not share common immunological determinants with H2b or any other core histone-contrary to the suggestion that it was an H2b-like protein (Ausio and Subirana, 1982). With H1 there was a crossreaction between S3 and anti-H1 as well as with H1 and anti-S3. Although similar to somatic H1, S3 is not identical with it. This fact makes S3 an interesting example of another protein of the H1-H5 type, present in a completely inactive chromatin.