Literature DB >> 3986710

Purification and chemical characterization of an exopolysaccharide isolated from Capnocytophaga ochracea.

J K Dyer, R W Bolton.   

Abstract

Purification and chemical characterization of an immunosuppressive exopolysaccharide from Capnocytophaga ochracea strain 25 are described. This polysaccharide was extracted from spent culture medium by cold ethanol precipitation. Purification was accomplished by trichloroacetic acid and pronase treatments in combination with diethylaminoethyl-Sepharose and concanavalin A-Sepharose chromatography. Purity of the exopolysaccharide was ascertained by polyacrylamide gel electrophoresis using periodic acid--Schiff staining. The exopolysaccharide was free of protein, nucleic acid, and lipopolysaccharide, but contained large amounts of mannose with lesser quantities of glucose, galactose, glucuronic acid, and glucosamine.

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Year:  1985        PMID： 3986710     DOI： 10.1139/m85-001

Source DB:  PubMed          Journal:  Can J Microbiol        ISSN： 0008-4166            Impact factor:   2.419


  7 in total

1.  Production of an Extracellular Polysaccharide by Haloferax mediterranei.

Authors:  J Antón; I Meseguer; F Rodríguez-Valera
Journal:  Appl Environ Microbiol       Date:  1988-10       Impact factor: 4.792

2.  Involvement of the Type IX Secretion System in Capnocytophaga ochracea Gliding Motility and Biofilm Formation.

Authors:  Daichi Kita; Satoshi Shibata; Yuichiro Kikuchi; Eitoyo Kokubu; Koji Nakayama; Atsushi Saito; Kazuyuki Ishihara
Journal:  Appl Environ Microbiol       Date:  2016-01-04       Impact factor: 4.792

3.  Lipopolysaccharidelike immunological properties of cell wall glycoproteins isolated from Cytophaga johnsonae.

Authors:  D M de Jong; J L Pate; T N Kirkland; C E Taylor; P J Baker; K Takayama
Journal:  Infect Immun       Date:  1991-08       Impact factor: 3.441

4.  Comparison of the virulence of exopolysaccharide-producing Prevotella intermedia to exopolysaccharide non-producing periodontopathic organisms.

Authors:  Takeshi Yamanaka; Kazuyoshi Yamane; Tomoyo Furukawa; Chiho Matsumoto-Mashimo; Chieko Sugimori; Takayuki Nambu; Noboru Obata; Clay B Walker; Kai-Poon Leung; Hisanori Fukushima
Journal:  BMC Infect Dis       Date:  2011-08-25       Impact factor: 3.090

5.  Pathogenicity of exopolysaccharide-producing Actinomyces oris isolated from an apical abscess lesion.

Authors:  K Yamane; T Nambu; T Yamanaka; K Ishihara; T Tatami; C Mashimo; C B Walker; K-P Leung; H Fukushima
Journal:  Int Endod J       Date:  2012-08-17       Impact factor: 5.264

6.  Gene expression profile and pathogenicity of biofilm-forming Prevotella intermedia strain 17.

Authors:  Takeshi Yamanaka; Tomoyo Furukawa; Chiho Matsumoto-Mashimo; Kazuyoshi Yamane; Chieko Sugimori; Takayuki Nambu; Naoki Mori; Hiroyuki Nishikawa; Clay B Walker; Kai-Poon Leung; Hisanori Fukushima
Journal:  BMC Microbiol       Date:  2009-01-16       Impact factor: 3.605

7.  Involvement of luxS in Biofilm Formation by Capnocytophaga ochracea.

Authors:  Kyoko Hosohama-Saito; Eitoyo Kokubu; Kazuko Okamoto-Shibayama; Daichi Kita; Akira Katakura; Kazuyuki Ishihara
Journal:  PLoS One       Date:  2016-01-22       Impact factor: 3.240
  7 in total

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