Temporal relationships of latency and magnitudes of Limulus ventral photoreceptor potentials elicited by two light pulses.

As the dark interval between two stimulating light pulses increases, the latent period of the ensuing receptor potential increases to an asymptote reached about midway during the latency and is, thereafter, constant. The latent period is thus divisible into two segments, the first of which is light sensitive and the second not. The possible significance of these segments has been explored [12]. During the time the latent period is increasing toward its limiting value, the magnitudes of both components of the receptor potential are constant and maximal. The magnitude of the first component of the receptor potential begins to decline at a pulse interval of 15 msec, signify that the contribution of photons incident on the photoreceptor from the second pulse is diminishing. The magnitude of the second component of the receptor potential begins to decline at a pulse interval of 20 msec, suggesting that the contribution of photons delivered by the first pulse is diminishing. These results demonstrate that the two pulse stimulating paradigm reveals two segments of the receptor potential latent period and the ability of the photoreceptor to integrate radiant energy incident during the latent period, as measured by receptor potential magnitude.