Literature DB >> 3985968

Purification and properties of gamma-oxalomesaconate hydratase from Pseudomonas ochraceae grown with phthalate.

K Maruyama.   

Abstract

Pseudomonas ochraceae produced inducibly a hydro-lyase which catalyzes the reversible conversion of gamma-oxalomesaconate into (-)-gamma-oxalocitramalate. The enzyme has been purified to homogeneity from the bacteria grown with phthalate. The enzyme was a dimeric protein (pI=4.9) with a Mr of 68,000 and showed a high specificity for gamma-oxalomesaconate (Km=14 microM) and (-)-gamma-oxalocitramalate (Km=6.4 microM). Equilibrium constant for the hydration of gamma-oxalomesaconate at pH 8.0 and 24 degrees C was 2.5. Various thiols activated the enzyme.

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Year:  1985        PMID： 3985968     DOI： 10.1016/0006-291x(85)91674-2

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  The 4-oxalomesaconate hydratase gene, involved in the protocatechuate 4,5-cleavage pathway, is essential to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6.

Authors:  H Hara; E Masai; Y Katayama; M Fukuda
Journal:  J Bacteriol       Date:  2000-12       Impact factor: 3.490

2.  Genetic and biochemical characterization of a 2-pyrone-4, 6-dicarboxylic acid hydrolase involved in the protocatechuate 4, 5-cleavage pathway of Sphingomonas paucimobilis SYK-6.

Authors:  E Masai; S Shinohara; H Hara; S Nishikawa; Y Katayama; M Fukuda
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

3.  Characterization of the 4-carboxy-4-hydroxy-2-oxoadipate aldolase gene and operon structure of the protocatechuate 4,5-cleavage pathway genes in Sphingomonas paucimobilis SYK-6.

Authors:  Hirofumi Hara; Eiji Masai; Keisuke Miyauchi; Yoshihiro Katayama; Masao Fukuda
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

4.  Regulatory system of the protocatechuate 4,5-cleavage pathway genes essential for lignin downstream catabolism.

Authors:  Naofumi Kamimura; Kazuhiro Takamura; Hirofumi Hara; Daisuke Kasai; Ryo Natsume; Toshiya Senda; Yoshihiro Katayama; Masao Fukuda; Eiji Masai
Journal:  J Bacteriol       Date:  2010-04-30       Impact factor: 3.490

5.  Plasmid-encoded phthalate catabolic pathway in Arthrobacter keyseri 12B.

Authors:  R W Eaton
Journal:  J Bacteriol       Date:  2001-06       Impact factor: 3.490

6.  Genetic and biochemical characterization of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase and its role in the protocatechuate 4,5-cleavage pathway in Sphingomonas paucimobilis SYK-6.

Authors:  E Masai; K Momose; H Hara; S Nishikawa; Y Katayama; M Fukuda
Journal:  J Bacteriol       Date:  2000-12       Impact factor: 3.490

7.  Characterization of the protocatechuate 4,5-cleavage pathway operon in Comamonas sp. strain E6 and discovery of a novel pathway gene.

Authors:  Naofumi Kamimura; Taichi Aoyama; Rieko Yoshida; Kenji Takahashi; Daisuke Kasai; Tomokuni Abe; Kohei Mase; Yoshihiro Katayama; Masao Fukuda; Eiji Masai
Journal:  Appl Environ Microbiol       Date:  2010-10-15       Impact factor: 4.792
  7 in total

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