Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Concentration and purification of influenza virus from allantoic fluid.

Literature DB >> 3985314

Concentration and purification of influenza virus from allantoic fluid.

D J Arora, P Tremblay, R Bourgault, S Boileau.

Abstract

A simple procedure which enables the concentration and purification of influenza virus, using an angular rotor, is described. Virus is concentrated over a sucrose step gradient. The same gradients are reused and volumes up to 4 liters are concentrated in 1 day. The concentrated virus is further purified by a simplified density-gradient technique. No host cell protein is detectable in the final product. The technique offers a broad application potential for concentrating and purifying other viruses.

Entities: Chemical Species

Mesh：

Year: 1985 PMID： 3985314 DOI： 10.1016/0003-2697(85)90103-4

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

Keyword Cloud
Cited

17 in total

1. Concentrating Autographa californica nuclear polyhedrosis virus and recombinant alkaline phosphatase from insect cells using a temperature-sensitive hydrogel.

Authors: J H Park; C H Park; I S Chung
Journal: Cytotechnology Date: 1997-11 Impact factor: 2.058

2. Complete sequences of the neuraminidase genes of swine influenza viruses (H1N1) associated with the respiratory disease in pigs.

Authors: D S Arora; P Tijssen; S Dea; M Henrichon
Journal: Virus Genes Date: 1997 Impact factor: 2.332

3. Type 1 responses of human Vγ9Vδ2 T cells to influenza A viruses.

Authors: Gang Qin; Yinping Liu; Jian Zheng; Iris H Y Ng; Zheng Xiang; Kwok-Tai Lam; Huawei Mao; Hong Li; J S Malik Peiris; Yu-Lung Lau; Wenwei Tu
Journal: J Virol Date: 2011-07-13 Impact factor: 5.103

4. Oligomeric structure of glycoproteins in herpes simplex virus type 1.

Authors: C G Handler; R J Eisenberg; G H Cohen
Journal: J Virol Date: 1996-09 Impact factor: 5.103

5. Characterization of infectious salmon anemia virus, an orthomyxo-like virus isolated from Atlantic salmon (Salmo salar L.).

Authors: K Falk; E Namork; E Rimstad; S Mjaaland; B H Dannevig
Journal: J Virol Date: 1997-12 Impact factor: 5.103

6. GM-CSF modulates pulmonary resistance to influenza A infection.

Authors: Zvjezdana Sever-Chroneos; Aditi Murthy; Jeremy Davis; Jon Matthew Florence; Anna Kurdowska; Agnieszka Krupa; Jay W Tichelaar; Mitchell R White; Kevan L Hartshorn; Lester Kobzik; Jeffrey A Whitsett; Zissis C Chroneos
Journal: Antiviral Res Date: 2011-09-08 Impact factor: 5.970

Concentration and purification of influenza virus from allantoic fluid.

1. Concentrating Autographa californica nuclear polyhedrosis virus and recombinant alkaline phosphatase from insect cells using a temperature-sensitive hydrogel.

2. Complete sequences of the neuraminidase genes of swine influenza viruses (H1N1) associated with the respiratory disease in pigs.

3. Type 1 responses of human Vγ9Vδ2 T cells to influenza A viruses.

4. Oligomeric structure of glycoproteins in herpes simplex virus type 1.

5. Characterization of infectious salmon anemia virus, an orthomyxo-like virus isolated from Atlantic salmon (Salmo salar L.).

6. GM-CSF modulates pulmonary resistance to influenza A infection.

7. Genetic variation in swine influenza virus A isolate associated with proliferative and necrotizing pneumonia in pigs.

8. Evidence for a protective role of pulmonary surfactant protein D (SP-D) against influenza A viruses.

9. Potent anti-influenza activity of cyanovirin-N and interactions with viral hemagglutinin.

10. Induction of long-term protective immune responses by influenza H5N1 virus-like particles.