Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Elimination of micronuclear specific DNA sequences early in anlagen development.

Literature DB >> 3982421

Elimination of micronuclear specific DNA sequences early in anlagen development.

Abstract

After conjugation in Tetrahymena thermophila, the old macronuclei degenerate, and new macronuclei (anlagen) develop. During anlagen development a number of DNA sequences found in the micronuclear genome (micronuclear limited sequences) are eliminated from the anlagen. A cloned copy of a repetitive micronuclear limited sequence has been used to determine the developmental stage at which micronuclear limited sequences are eliminated. DNAs from anlagen of various developmental stages were examined by Southern analysis. It was found that micronuclear limited sequences are present in 4C anlagen and essentially absent in 8C and 16C anlagen. The precipitous loss of these sequences in the 8C anlagen rules out under-replication as the mechanism for the loss and suggests that these sequences are specifically degraded early during anlagen development.

Entities: Species

Mesh：

Substances：
DNA

Year: 1985 PMID： 3982421 PMCID： PMC366682 DOI： 10.1128/mcb.5.1.93-98.1985

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

28 in total

Review 3. The DNA of ciliated protozoa.

Authors: D M Prescott
Journal: Microbiol Rev Date: 1994-06

4. Tetrahymena thermophila mutants defective in the developmentally programmed maturation and maintenance of the rDNA minichromosome.

Authors: G M Kapler; E Orias; E H Blackburn
Journal: Genetics Date: 1994-06 Impact factor: 4.562

5. A small family of elements with long inverted repeats is located near sites of developmentally regulated DNA rearrangement in Tetrahymena thermophila.

Authors: J M Wells; J L Ellingson; D M Catt; P J Berger; K M Karrer
Journal: Mol Cell Biol Date: 1994-09 Impact factor: 4.272

10. Deletion of the Tetrahymena thermophila rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome.

Authors: J S Yakisich; G M Kapler
Journal: Nucleic Acids Res Date: 2006-01-30 Impact factor: 16.971

10 in total

Elimination of micronuclear specific DNA sequences early in anlagen development.

1. Unequal gene amplification and transcription in the macronucleus of Tetrahymena pyriformis.

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

3. Rapid equilibrium isopycnic CsC1 gradients.

4. Single extrachromosomal ribosomal RNA gene copies are synthesized during amplification of the rDNA in Tetrahymena.

5. Tandemly repeated hexanucleotide at Tetrahymena rDNA free end is generated from a single copy during development.

6. Ribosomal RNA gene amplification in Tetrahymena may be associated with chromosome breakage and DNA elimination.

Review 7. Genome organization and reorganization in Tetrahymena.

8. Life cycle variation and regulation of macronuclear DNA content in Tetrahymena thermophila.

9. Reorganization of unique and repetitive sequences during nuclear development in Tetrahymena thermophila.

10. Allele-specific, selective amplification of a ribosomal RNA gene in Tetrahymena thermophila.

1. Diverse sequences within Tlr elements target programmed DNA elimination in Tetrahymena thermophila.

2. A family of DNA sequences is reproducibly rearranged in the somatic nucleus of Tetrahymena.

Review 3. The DNA of ciliated protozoa.

4. Tetrahymena thermophila mutants defective in the developmentally programmed maturation and maintenance of the rDNA minichromosome.

5. A small family of elements with long inverted repeats is located near sites of developmentally regulated DNA rearrangement in Tetrahymena thermophila.

6. Characterization of the macronuclear DNA of different species of Tetrahymena.

7. Nucleotide sequence structure and consistency of a developmentally regulated DNA deletion in Tetrahymena thermophila.

8. A developmentally regulated gene, ASI2, is required for endocycling in the macronuclear anlagen of Tetrahymena.

9. Macronuclear DNA molecules of Tetrahymena thermophila.

10. Deletion of the Tetrahymena thermophila rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome.