Literature DB >> 3977918

Superior resolution of gamma-crystallins from microdissected eye lens by cation-exchange high-performance liquid chromatography.

R J Siezen, E D Kaplan, R D Anello.   

Abstract

A novel procedure is presented for the rapid quantitative analysis of eye lens gamma-crystallins and beta s-crystallin by ion-exchange high-performance liquid chromatography on Synchropak CM300. At least six different gamma-crystallin gene products can be resolved from the soluble fraction of calf lens extract. This method is applicable to the analysis of microsections from individual lenses, and can be used to rapidly characterize spatial variations in gamma-crystallin composition which occur with aging and cataractogenesis.

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Year:  1985        PMID： 3977918     DOI： 10.1016/s0006-291x(85)80138-8

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  5 in total

1.  Binary-liquid phase separation of lens protein solutions.

Authors:  M L Broide; C R Berland; J Pande; O O Ogun; G B Benedek
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-01       Impact factor: 11.205

2.  Suppression of phase separation in solutions of bovine gamma IV-crystallin by polar modification of the sulfur-containing amino acids.

Authors:  J Pande; C Berland; M Broide; O Ogun; J Melhuish; G Benedek
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-01       Impact factor: 11.205

3.  Phase separation in aqueous solutions of lens gamma-crystallins: special role of gamma s.

Authors:  C Liu; N Asherie; A Lomakin; J Pande; O Ogun; G B Benedek
Journal:  Proc Natl Acad Sci U S A       Date:  1996-01-09       Impact factor: 11.205

4.  Human lens gamma-crystallins: isolation, identification, and characterization of the expressed gene products.

Authors:  R J Siezen; J A Thomson; E D Kaplan; G B Benedek
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205

5.  Oxidation of gamma II-crystallin solutions yields dimers with a high phase separation temperature.

Authors:  J Pande; A Lomakin; B Fine; O Ogun; I Sokolinski; G Benedek
Journal:  Proc Natl Acad Sci U S A       Date:  1995-02-14       Impact factor: 11.205
  5 in total

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