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Literature DB >> 3977873

Starvation and feeding with a high-carbohydrate diet induce changes in the specific activity of rat hepatic pyruvate kinase.

Abstract

A highly specific radiosandwich assay for hepatic type-L pyruvate kinase (PK) determinants was developed. This assay was used to evaluate changes in PK specific activity which occur on starvation or feeding with a high-carbohydrate diet. Despite a large increase in both catalytic activity and immunoreactive protein, the calculated specific activity falls on feeding, and the opposite effect occurs on starvation. These change can be attributed to disproportionate changes in immunoreactive protein compared with changes in catalytic activity.

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Year: 1985 PMID： 3977873 PMCID： PMC1144705 DOI： 10.1042/bj2260299

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

16 in total

1. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

2. Batch preparation of rabbit gammaG globulin with deae-cellulose.

Authors: A E Reif
Journal: Immunochemistry Date: 1969-09

3. Hormonal regulation of liver pyruvate kinase concentration and activity.

Authors: M L Johnson; C M Veneziale
Journal: Biochemistry Date: 1980-05-13 Impact factor: 3.162

4. Studies on the biosynthesis of hepatic pyruvate kinase and its correlation with enhanced hepatic lipogenesis in meal-trained rats.

Authors: T J Hopkirk; D P Bloxham
Journal: Biochem J Date: 1979-08-15 Impact factor: 3.857

1. Metabolic adaptation of the renal carbohydrate metabolism. I. Effects of starvation on the gluconeogenic and glycolytic fluxes in the proximal and distal renal tubules.

Authors: L García-Salguero; J A Lupiáñez
Journal: Mol Cell Biochem Date: 1988-10 Impact factor: 3.396

1 in total

Starvation and feeding with a high-carbohydrate diet induce changes in the specific activity of rat hepatic pyruvate kinase.

1. Protein measurement with the Folin phenol reagent.

2. Batch preparation of rabbit gammaG globulin with deae-cellulose.

3. Hormonal regulation of liver pyruvate kinase concentration and activity.

4. Studies on the biosynthesis of hepatic pyruvate kinase and its correlation with enhanced hepatic lipogenesis in meal-trained rats.

5. Alteration in liver pyruvate kinase protein and catalytic acitivity upon starvation and refeeding.

6. Hepatic pyruvate kinase. Quantitative measurements of phosphorylation in vitro and in the isolated rat hepatocyte.

7. Cotranslation of L and L' pyruvate kinase messenger RNAs from human fetal liver.

8. Glucagon-stimulated phosphorylation of pyruvate kinase in hepatocytes.

9. Long-term modulation of type L pyruvate kinase activity in young and mature rats.

10. Insulin and dexamethasone effects on liver fructose bisphosphatase.

1. Metabolic adaptation of the renal carbohydrate metabolism. I. Effects of starvation on the gluconeogenic and glycolytic fluxes in the proximal and distal renal tubules.