| Literature DB >> 3973395 |
Abstract
A method is described in which enzyme amplification is used for the quantitative determination of placental alkaline phosphatase. In this particular application, placental alkaline phosphatase is captured on a solid surface by means of a monoclonal antibody. The enzyme is then determined by dephosphorylation of NADP to NAD which activates a strictly NAD-dependent redox cycle leading to the formation of a coloured product.Entities:
Mesh:
Substances:
Year: 1985 PMID: 3973395 DOI: 10.1016/0022-1759(85)90316-3
Source DB: PubMed Journal: J Immunol Methods ISSN: 0022-1759 Impact factor: 2.303