Literature DB >> 3968988

A colorimetric assay of pancreatic lipase: rapid detection of lipase and colipase separated by gel filtration.

J Roberts, V J Stella, C J Decedue.   

Abstract

A rapid assay for pancreatic lipase (E.C., glycerol-ester hydrolase 3.1.1.3) is described. The assay is based on the color change of a pH indicator as butyric acid is released from the substrate tributyrin. A mixture made with tributyrin and the water soluble components of the assay is ideally suited for use as a rapid test as, for example, when assaying chromatography fractions. Quantitative data can be obtained by measuring the disappearance of absorbance at 557 nm versus a blank reaction. The assay has been used in the rapid preparation of colipase-free lipase and colipase.

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Year:  1985        PMID： 3968988     DOI： 10.1007/bf02534361

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  9 in total

1.  On human pancreatic triacylglycerol lipase: isolation and some properties.

Authors:  A Vandermeers; M C Vandermeers-Piret; J Rathé; J Christophe
Journal:  Biochim Biophys Acta       Date:  1974-11-25

2.  Pancreatic lipase and co-lipase. Interactions and effects of bile salts and other detergents.

Authors:  B Borgström; C Erlanson
Journal:  Eur J Biochem       Date:  1973-08-01

3.  Pancreatic juice co-lipase: physiological importance.

Authors:  B Borgström; C Erlanson
Journal:  Biochim Biophys Acta       Date:  1971-08-20

4.  The effect of sodium taurodesoxycholate and pH on the gel filtration behavior of rat pancreatic protein and lipases.

Authors:  R G Morgan; J Barrowman; B Borgström
Journal:  Biochim Biophys Acta       Date:  1969-02-04

5.  Purification from porcine pancreas of two molecular species with lipase activity.

Authors:  R Verger; G H de Haas; L Sarda
Journal:  Biochim Biophys Acta       Date:  1969

6.  Immobilized pancreatic lipase and colipase for purification and binding studies.

Authors:  J S Patton; L Andersson
Journal:  FEBS Lett       Date:  1978-02-15       Impact factor: 4.124

7.  Binding of porcine pancreatic lipase and colipase in the absence of substrate studies by two-phase partition and affinity chromatography.

Authors:  J S Patton; P A Albertsson; C Erlanson; B Borgström
Journal:  J Biol Chem       Date:  1978-06-25       Impact factor: 5.157

8.  Studies on the effect of bile salt and colipase on enzymatic lipolysis. Improved method for the determination of pancreatic lipase and colipase.

Authors:  J Rathelot; R Julien; P Canioni; C Coeroli; L Sarda
Journal:  Biochimie       Date:  1975       Impact factor: 4.079

9.  Lipase-colipase interactions during gel filtration. High and low affinity binding situations.

Authors:  J S Patton; J Donnér; B Borgström
Journal:  Biochim Biophys Acta       Date:  1978-04-28
  9 in total
  1 in total

1.  A novel spectrophotometric assay for lipase activity utilizing cis-parinaric acid.

Authors:  A M Rogel; W L Stone; F O Adebonojo
Journal:  Lipids       Date:  1989-06       Impact factor: 1.880
  1 in total

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