Literature DB >> 3968189

Isolation of pituitary fibroblast growth factor by fast protein liquid chromatography (FPLC): partial chemical and biological characterization.

D Gospodarowicz, S Massoglia, J Cheng, G M Lui, P Böhlen.   

Abstract

Bovine pituitary fibroblast growth factor has been purified 222,000-fold to homogeneity by a combination of differential salt extraction, gel filtration, and ion exchange chromatography on Mono S column. Pituitary FGF is a single-chain polypeptide with an apparent molecular mass of 15,800 and an isoelectric point of 9.6. It is highly active in triggering the proliferation of bovine and human vascular endothelial cell [half-maximal stimulation at 23-40 pg/ml (1.5-2.6 pM) and saturation between 140 and 280 pg/ml (9.3-18.6 pM)]. It displays a similar activity on bovine vascular smooth muscle cells, corneal endothelial cells, granulosa and adrenal cortex cells, and rabbit costal chondrocytes.

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Year:  1985        PMID： 3968189     DOI： 10.1002/jcp.1041220223

Source DB:  PubMed          Journal:  J Cell Physiol        ISSN： 0021-9541            Impact factor:   6.384


  18 in total

1.  A modified chorioallantoic membrane (CAM) assay for qualitative and quantitative study of growth factors. Studies on the effects of carriers, PBS, angiogenin, and bFGF.

Authors:  J Wilting; B Christ; M Bokeloh
Journal:  Anat Embryol (Berl)       Date:  1991

2.  Endothelial stimulation of intimal cell proliferation in a porcine aortic organ culture.

Authors:  E W Koo; A I Gotlieb
Journal:  Am J Pathol       Date:  1989-03       Impact factor: 4.307

3.  Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF.

Authors:  F Esch; A Baird; N Ling; N Ueno; F Hill; L Denoroy; R Klepper; D Gospodarowicz; P Böhlen; R Guillemin
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

4.  Cultured human foreskin fibroblasts produce a factor that stimulates their growth with properties similar to basic fibroblast growth factor.

Authors:  M T Story
Journal:  In Vitro Cell Dev Biol       Date:  1989-05

5.  Acidic fibroblast growth factor enhances regeneration of processes by postnatal mammalian retinal ganglion cells in culture.

Authors:  S A Lipton; J A Wagner; R D Madison; P A D'Amore
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205

6.  Tumor vascular permeability factor stimulates endothelial cell growth and angiogenesis.

Authors:  D T Connolly; D M Heuvelman; R Nelson; J V Olander; B L Eppley; J J Delfino; N R Siegel; R M Leimgruber; J Feder
Journal:  J Clin Invest       Date:  1989-11       Impact factor: 14.808

7.  Induction of vascular endothelial tubular morphogenesis by human glioma cells. A model system for tumor angiogenesis.

Authors:  T Abe; K Okamura; M Ono; K Kohno; T Mori; S Hori; M Kuwano
Journal:  J Clin Invest       Date:  1993-07       Impact factor: 14.808

8.  The human fibroblast growth factor receptor genes: a common structural arrangement underlies the mechanisms for generating receptor forms that differ in their third immunoglobulin domain.

Authors:  D E Johnson; J Lu; H Chen; S Werner; L T Williams
Journal:  Mol Cell Biol       Date:  1991-09       Impact factor: 4.272

9.  Regulation of protein metabolism and DNA synthesis by fibroblast growth factor in BHK-21 cells.

Authors:  M Ross; F J Ballard
Journal:  Biochem J       Date:  1988-01-15       Impact factor: 3.857

10.  Platelet derived growth factor and fibroblast growth factor basic levels in the vitreous of patients with vitreoretinal disorders.

Authors:  L Cassidy; P Barry; C Shaw; J Duffy; S Kennedy
Journal:  Br J Ophthalmol       Date:  1998-02       Impact factor: 4.638
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