| Literature DB >> 3968044 |
Abstract
A phosphorylated 2-keto-3-deoxyoctonic acid (KDO) was released from the lipopolysaccharides of Vibrio cholerae Ogawa and Inaba after strong acid hydrolysis. The phosphorylated KDO was identified by gas-liquid chromatography and mass spectrometry after reduction and permethylation as KDO-5-phosphate and an isomer of it being phosphorylated at position 7 or 8. After treatment with alkaline phosphatase, KDO was detected by gas-liquid chromatography and mass spectrometry. It was indistinguishable from authentic 2-keto-3-deoxy-D-manno-octonic acid.Entities:
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Year: 1985 PMID: 3968044 PMCID: PMC214959 DOI: 10.1128/jb.161.2.795-798.1985
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490