Literature DB >> 3968041

Efficient transformation of Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.

J C Hinton, M C Perombelon, G P Salmond.   

Abstract

We used a modified version of the method of Hanahan (D. Hanahan, J. Mol. Biol. 166:557-580, 1983) to transform Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica with the plasmids pBR322, pBR325, and pAT153. The transformation frequency ranged from 1 X 10(2) to 4 X 10(4) colonies per micrograms of plasmid DNA. The nature of these transformants was confirmed by plasmid analysis. ColE1-based plasmids make potentially useful cloning vectors for the study of genes involved in the pathogenesis of this species.

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Year:  1985        PMID： 3968041      PMCID： PMC214956          DOI： 10.1128/jb.161.2.786-788.1985

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  16 in total

1.  Genetic studies with heteroduplex DNA of bacteriophage fl. Asymmetric segregation, base correction and implications for the mechanism of genetic recombination.

Authors:  V Enea; G F Vovis; N D Zinder
Journal:  J Mol Biol       Date:  1975-08-15       Impact factor: 5.469

Review 2.  Transfection of Enterobacteriaceae and its applications.

Authors:  R Benzinger
Journal:  Microbiol Rev       Date:  1978-03

3.  Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.

Authors:  J B Hansen; R H Olsen
Journal:  J Bacteriol       Date:  1978-07       Impact factor: 3.490

4.  Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.

Authors:  F Bolivar
Journal:  Gene       Date:  1978-10       Impact factor: 3.688

5.  Frozen-thawed bacteria as recipients of isolated coliphage DNA.

Authors:  S Y Dityatkin; K V Lisovskaya; N N Panzhava; B N Iliashenko
Journal:  Biochim Biophys Acta       Date:  1972-10-27

6.  Transformation and preservation of competent bacterial cells by freezing.

Authors:  D A Morrison
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

7.  Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.

Authors:  D B Clewell; D R Helinski
Journal:  Biochemistry       Date:  1970-10-27       Impact factor: 3.162

8.  Recombination between bacterial plasmids leading to the formation of plasmid multimers.

Authors:  J R Bedbrook; F M Ausubel
Journal:  Cell       Date:  1976-12       Impact factor: 41.582

9.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

10.  Trans-complementable copy-number mutants of plasmid ColE1.

Authors:  A J Twigg; D Sherratt
Journal:  Nature       Date:  1980-01-10       Impact factor: 49.962
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  14 in total

1.  Two Genomic Regions Involved in Catechol Siderophore Production by Erwinia carotovora.

Authors:  C T Bull; C A Ishimaru; J E Loper
Journal:  Appl Environ Microbiol       Date:  1994-02       Impact factor: 4.792

2.  Bacteriophage lambda-mediated transposon mutagenesis of phytopathogenic and epiphytic Erwinia species is strain dependent.

Authors:  F M Ellard; A Cabello; G P Salmond
Journal:  Mol Gen Genet       Date:  1989-09

3.  AepA of Pectobacterium is not involved in the regulation of extracellular plant cell wall degrading enzymes production.

Authors:  Viia Kõiv; Liis Andresen; Andres Mäe
Journal:  Mol Genet Genomics       Date:  2010-04-13       Impact factor: 3.291

4.  High-affinity iron uptake systems present in Erwinia carotovora subsp. carotovora include the hydroxamate siderophore aerobactin.

Authors:  C A Ishimaru; J E Loper
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

5.  Identification and cloning of an Erwinia carotovora subsp. carotovora bacteriocin regulator gene by insertional mutagenesis.

Authors:  D Y Chuang; A G Kyeremeh; Y Gunji; Y Takahara; Y Ehara; T Kikumoto
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

6.  Cloning and expression of the Erwinia carotovora subsp. carotovora gene encoding the low-molecular-weight bacteriocin carocin S1.

Authors:  Duen-yau Chuang; Yung-chei Chien; Huang-Pin Wu
Journal:  J Bacteriol       Date:  2006-10-27       Impact factor: 3.490

7.  Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors.

Authors:  K S Bell; M Sebaihia; L Pritchard; M T G Holden; L J Hyman; M C Holeva; N R Thomson; S D Bentley; L J C Churcher; K Mungall; R Atkin; N Bason; K Brooks; T Chillingworth; K Clark; J Doggett; A Fraser; Z Hance; H Hauser; K Jagels; S Moule; H Norbertczak; D Ormond; C Price; M A Quail; M Sanders; D Walker; S Whitehead; G P C Salmond; P R J Birch; J Parkhill; I K Toth
Journal:  Proc Natl Acad Sci U S A       Date:  2004-07-19       Impact factor: 11.205

8.  Marker-exchange mutagenesis of a pectate lyase isozyme gene in Erwinia chrysanthemi.

Authors:  D L Roeder; A Collmer
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

9.  Molecular analysis of the major cellulase (CelV) of Erwinia carotovora: evidence for an evolutionary "mix-and-match" of enzyme domains.

Authors:  V J Cooper; G P Salmond
Journal:  Mol Gen Genet       Date:  1993-11

10.  Extracellular secretion of Carocin S1 in Pectobacterium carotovorum subsp. carotovorum occurs via the type III secretion system integral to the bacterial flagellum.

Authors:  Yung-Chieh Chan; Huang-Pin Wu; Duen-Yau Chuang
Journal:  BMC Microbiol       Date:  2009-08-27       Impact factor: 3.605
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