Literature DB >> 3963384

Detection and quantitation of cellulase by Congo red staining of substrates in a cup-plate diffusion assay.

J H Carder.   

Abstract

A simple, inexpensive, and sensitive cellulase assay which allows simultaneous enzyme quantitation for many samples has been developed. Radial diffusion of enzyme into substrate incorporated in an agar gel produces zones of hydrolysis which can be visualized by staining undegraded substrate with Congo red dye. Comparisons were made between this and other commonly used cellulase assays based on accuracy, sensitivity, and ease of use.

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Year:  1986        PMID: 3963384     DOI: 10.1016/0003-2697(86)90063-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  18 in total

1.  Purification and characterization of an endoglucanase from Streptomyces lividans 66 and DNA sequence of the gene.

Authors:  M Théberge; P Lacaze; F Shareck; R Morosoli; D Kluepfel
Journal:  Appl Environ Microbiol       Date:  1992-03       Impact factor: 4.792

2.  The nematicide Serratia plymuthica M24T3 colonizes Arabidopsis thaliana, stimulates plant growth, and presents plant beneficial potential.

Authors:  Diogo Neves Proença; Stefan Schwab; Márcia Soares Vidal; José Ivo Baldani; Gustavo Ribeiro Xavier; Paula V Morais
Journal:  Braz J Microbiol       Date:  2019-06-08       Impact factor: 2.476

3.  The distribution of extracellular cellulase activity in marine Eukaryotes, thraustochytrids.

Authors:  Naoki Nagano; Shou Matsui; Tomoyo Kuramura; Yousuke Taoka; Daiske Honda; Masahiro Hayashi
Journal:  Mar Biotechnol (NY)       Date:  2010-05-05       Impact factor: 3.619

4.  An ascomycota coculture in batch bioreactor is better than polycultures for cellulase production.

Authors:  Christian Hernández; Adriane M F Milagres; Gerardo Vázquez-Marrufo; Karla María Muñoz-Páez; José Antonio García-Pérez; Enrique Alarcón
Journal:  Folia Microbiol (Praha)       Date:  2018-02-08       Impact factor: 2.099

5.  Induction of a Cryphonectria parasitica cellobiohydrolase I gene is suppressed by hypovirus infection and regulated by a GTP-binding-protein-linked signaling pathway involved in fungal pathogenesis.

Authors:  P Wang; D L Nuss
Journal:  Proc Natl Acad Sci U S A       Date:  1995-12-05       Impact factor: 11.205

6.  Cloning of an Azorhizobium caulinodans endoglucanase gene and analysis of its role in symbiosis.

Authors:  D Geelen; M van Montagu; M Holsters
Journal:  Appl Environ Microbiol       Date:  1995-09       Impact factor: 4.792

7.  Differential ethylene-inducible expression of cellulase in pepper plants.

Authors:  L Ferrarese; L Trainotti; P Moretto; P Polverino de Laureto; N Rascio; G Casadoro
Journal:  Plant Mol Biol       Date:  1995-11       Impact factor: 4.076

8.  PECTOPLATE: the simultaneous phenotyping of pectin methylesterases, pectinases, and oligogalacturonides in plants during biotic stresses.

Authors:  Vincenzo Lionetti
Journal:  Front Plant Sci       Date:  2015-05-13       Impact factor: 5.753

9.  Thiol reductive stress induces cellulose-anchored biofilm formation in Mycobacterium tuberculosis.

Authors:  Abhishek Trivedi; Parminder Singh Mavi; Deepak Bhatt; Ashwani Kumar
Journal:  Nat Commun       Date:  2016-04-25       Impact factor: 14.919

10.  Mating type-dependent partner sensing as mediated by VEL1 in Trichoderma reesei.

Authors:  Hoda Bazafkan; Christoph Dattenböck; Stefan Böhmdorfer; Doris Tisch; Eva Stappler; Monika Schmoll
Journal:  Mol Microbiol       Date:  2015-04-16       Impact factor: 3.501

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