Literature DB >> 3963380

Purification, solubility, and pKa of veratridine.

L C McKinney, S Chakraverty, P De Weer.   

Abstract

The alkaloid neurotoxin veratridine is widely used by cell physiologists to increase membrane sodium permeability. The compound is only sporadically available from commercial sources, but can be purified (Kupchan et al., 1953, J. Amer. Chem. Soc. 75, 5519-5524) from veratrine, a mixture of several alkaloids. We describe here a purification procedure only slightly modified from that of Kupchan et al., and include important details not mentioned in the original paper. Ultraviolet and infrared spectra are presented. We have also determined the pKa and solubility of veratridine in 150 mM NaCl at 25 degrees C. The solubility is steeply pH dependent, ranging from 0.61 +/- 0.02 mM above pH 12 to 18.5 mM at pH 8.07. The pKa, determined from the solubility versus pH curve, was found to be 9.54 +/- 0.02.

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Year:  1986        PMID： 3963380     DOI： 10.1016/0003-2697(86)90056-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  Antagonism by local anesthetics of sodium channel activators in the presence of scorpion toxins: two mechanisms for competitive inhibition.

Authors:  Stanley Lee Son; Kin Wong; Gary Strichartz
Journal:  Cell Mol Neurobiol       Date:  2004-08       Impact factor: 5.046

2.  Point mutations in segment I-S6 render voltage-gated Na+ channels resistant to batrachotoxin.

Authors:  S Y Wang; G K Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-03       Impact factor: 11.205

3.  Late cardiac sodium current can be assessed using automated patch-clamp.

Authors:  Morgan Chevalier; Bogdan Amuzescu; Vaibhavkumar Gawali; Hannes Todt; Thomas Knott; Olaf Scheel; Hugues Abriel
Journal:  F1000Res       Date:  2014-10-16

4.  Relation between veratridine reaction dynamics and macroscopic Na current in single cardiac cells.

Authors:  X G Zong; M Dugas; P Honerjäger
Journal:  J Gen Physiol       Date:  1992-05       Impact factor: 4.086

5.  Mutually exclusive action of cationic veratridine and cevadine at an intracellular site of the cardiac sodium channel.

Authors:  P Honerjäger; M Dugas; X G Zong
Journal:  J Gen Physiol       Date:  1992-05       Impact factor: 4.086

6.  Supramolecular Complexes of Plant Neurotoxin Veratridine with Cyclodextrins and Their Antidote-like Effect on Neuro-2a Cell Viability.

Authors:  Laura A Uribe; Sandra Leonardo; Thorbjørn Terndrup Nielsen; Casper Steinmann; Mònica Campàs; Alex Fragoso
Journal:  Pharmaceutics       Date:  2022-03-09       Impact factor: 6.321
  6 in total

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