Literature DB >> 396224

Establishment of a human lymphoblastoid cell line with specific antibody production against group A streptococcal carbohydrate.

M Steinitz, I Seppälä, K Eichmann, G Klein.   

Abstract

A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epsetin Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosamine-coupled T4-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 micrograms/ml anti-A-CHO antibodies of the IgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 micrograms/ml of supernatant.

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Year:  1979        PMID： 396224

Source DB:  PubMed          Journal:  Immunobiology        ISSN： 0171-2985            Impact factor:   3.144


  8 in total

1.  Detection of a unique human V kappa IV germline gene by a cloned cDNA probe.

Authors:  P Marsh; F Mills; H Gould
Journal:  Nucleic Acids Res       Date:  1985-09-25       Impact factor: 16.971

2.  Construction and testing of mouse--human heteromyelomas for human monoclonal antibody production.

Authors:  N N Teng; K S Lam; F Calvo Riera; H S Kaplan
Journal:  Proc Natl Acad Sci U S A       Date:  1983-12       Impact factor: 11.205

Review 3.  Epstein-Barr virus (EBV), lymphocytes and transformation.

Authors:  J Zeuthen
Journal:  J Cancer Res Clin Oncol       Date:  1983       Impact factor: 4.553

Review 4.  Human monoclonal antibodies.

Authors:  S P Cole; B G Campling; T Atlaw; D Kozbor; J C Roder
Journal:  Mol Cell Biochem       Date:  1984-06       Impact factor: 3.396

5.  Human hybridomas constructed with antigen-specific Epstein-Barr virus-transformed cell lines.

Authors:  D Kozbor; A E Lagarde; J C Roder
Journal:  Proc Natl Acad Sci U S A       Date:  1982-11       Impact factor: 11.205

6.  Structure of the VH and VL segments of polyreactive and monoreactive human natural antibodies to HIV-1 and Escherichia coli beta-galactosidase.

Authors:  N Harindranath; H Ikematsu; A L Notkins; P Casali
Journal:  Int Immunol       Date:  1993-12       Impact factor: 4.823

7.  IgD/lambda plasmocytoma with immunoglobulin kappa light-chain genes in the germ-line configuration.

Authors:  G Hauke; J T Epplen; J Chluba; A Hollmann; H H Peter
Journal:  J Clin Immunol       Date:  1988-09       Impact factor: 8.317

Review 8.  Challenges in Antibody Development against Tn and Sialyl-Tn Antigens.

Authors:  Liliana R Loureiro; Mylène A Carrascal; Ana Barbas; José S Ramalho; Carlos Novo; Philippe Delannoy; Paula A Videira
Journal:  Biomolecules       Date:  2015-08-11
  8 in total

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