Incorporation of [35S]sulphate and [3H]thymidine into isolated bovine articular chondrocytes in vitro. Effect of autogenous conditioned synovial medium.

The present work is a part of a study series concerned with the metabolic events taking place in the synovial joint tissues. We have previously reported that synovial products not only enhance the degradation of articular cartilage proteoglycans but also inhibit the synthesis of articular cartilage proteoglycans, and that these cellular interactions may be partly modulated by arachidonic acid metabolites. In the experiments reported here we show that the addition of conditioned synovial medium to confluent monolayer cultures of articular chondrocytes not only inhibits the synthesis of proteoglycans, measured as the incorporation of radiosulphate into cartilage proteoglycans but also inhibits the synthesis of DNA, measured as the incorporation of radiothymidine into the chondrocytes, in a dose-dependent manner. In our culture system, calf knee-joint synovial tissue produces PGE, and it has been reported that previous exposure to PGE2 affects the ability of the cell to respond to that same hormone. In these experiments, previous exposure to conditioned synovial medium affected neither the inhibition of the incorporation of radiosulphate nor the inhibition of the incorporation of radiothymidine.