Nifedipine reversibly arrests mitosis in stamen hair cells of tradescantia.

Mitotic stamen hair cells of Tradescantia virginiana (cv. Zwanenburg Blue) become arrested in metaphase following a 30-min treatment with 10 to 100 microM nifedipine, a Ca2+-channel entry blocker. The time interval between nuclear envelope breakdown and anaphase onset in untreated cells is approximately 33 min +/- 4 min; nifedipine extends this "metaphase transit time" beyond 70 min. Nifedipine can be photoreversed in situ by exposure to 365 nm light. UV illumination inactivates the drug, its inhibitory effect on Ca2+ is abolished, and cells arrested in metaphase enter anaphase within 3 to 18 min of UV exposure if CaCl2 is present in the medium. The interval between UV illumination and anaphase onset is inversely related to the extracellular concentration of CaCl2. If CaCl2 is not added to the medium, the interval between UV exposure and anaphase onset is usually longer than 18 min. The sole addition of 100 microM CaCl2 to the medium is insufficient to reverse nifedipine inhibition; unless the cells are exposed to UV light, anaphase will not commence. The threshold concentration of free Ca2+ for rapid anaphase onset (less than 10 min after UV photoreversal) is between 1 and 10 microM. These results suggest that an influx of Ca2+ from the extracellular medium to the cytosolic compartment is necessary for normal progression from metaphase to anaphase and that this influx may serve as a trigger for chromosome separation.