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Literature DB >> 395023

Physiological characterization of polar Tn5-induced isoleucine-valine auxotrophs in Escherichia coli K.12: evidence for an internal promoter in the ilvOGEDA operon.

C M Berg, K J Shaw, J Vender, M Borucka-Mankiewicz.

Abstract

The properties of 22 isoleucine-valine auxotrophs induced in Escherichia coli K-12 by the transposable element, Tn5, were characterized on the basis of growth requirements, cross-feeding behavior, and enzyme activity. Mutants defective in ilvA, ilvC, ilvD and ilvE were found. Mutation in ilvE were not completely polar on ilvD and ilvA enzyme activities (that is, ilvE mutants possessed a low constitutive level of expression of the enzymes coded by ilvD and ilvA), while mutations in ilvD were completely polar on ilvA enzyme activity. The data suggest that there is an internal promoter between the sites of Tn5 insertion in ilvE and ilvD.

Entities: Chemical Species

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Year: 1979 PMID： 395023 PMCID： PMC1214080

Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562

11 in total

1. The effect of an ilvA Mu phage insertion on ilv gene expression in Escherichia coli K-12.

Authors: E L Kline; F T Bayliss
Journal: Biochem Biophys Res Commun Date: 1975-04-21 Impact factor: 3.575

2. Acetylornithinase of Escherichia coli: partial purification and some properties.

Authors: H J VOGEL; D M BONNER
Journal: J Biol Chem Date: 1956-01 Impact factor: 5.157

3. Transduction of linked genetic characters of the host by bacteriophage P1.

Authors: E S LENNOX
Journal: Virology Date: 1955-07 Impact factor: 3.616

4. The ilvEDA operon of Escherichia coli K12 encodes only one valine-alpha-ketoglutarate transaminase activity.

Authors: C W Adams; R P Lawther; G W Hatfield
Journal: Biochem Biophys Res Commun Date: 1979-07-27 Impact factor: 3.575

5. Regulation of isoleucine-valine biosynthesis in an ilvDAC deletion strain of Escherichia coli K-12.

Authors: E L Kline; C S Brown; W G Coleman; H E Umbarger
Journal: Biochem Biophys Res Commun Date: 1974-04-23 Impact factor: 3.575

6. Regulation of transaminase C synthesis in Escherichia coli: conditional leucine auxotrophy.

Authors: D McGilvray; H E Umbarger
Journal: J Bacteriol Date: 1974-11 Impact factor: 3.490

7. Polarity and the regulation of the ilv gene cluster in Escherichia coli strain K-12.

Authors: J M Smith; D E Smolin; H E Umbarger
Journal: Mol Gen Genet Date: 1976-10-18

8. Multivalent regulation of isoleucine-valine transaminase in an Escherichia coli K-12 ilvA deletion strain.

Authors: E L Kline; D N Manross; M L Warwick
Journal: J Bacteriol Date: 1977-05 Impact factor: 3.490

9. Transaminase B from Escherichia coli: quaternary structure, amino-terminal sequence, substrate specificity, and absence of a separate valine-alpha-ketoglutarate activity.

Authors: F C Lee-Peng; M A Hermodson; G B Kohlhaw
Journal: J Bacteriol Date: 1979-08 Impact factor: 3.490

10. Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5.

Authors: K J Shaw; C M Berg
Journal: Genetics Date: 1979-07 Impact factor: 4.562

30 in total

1. Further evidence that transposition of Tn5 in Escherichia coli is strongly enhanced by constitutively activated RecA proteins.

Authors: C T Kuan; I Tessman
Journal: J Bacteriol Date: 1992-11 Impact factor: 3.490

2. Multiple transcripts encoded by the ilvGMEDA gene cluster of Escherichia coli K-12.

Authors: F Huang; G Coppola; D H Calhoun
Journal: J Bacteriol Date: 1992-08 Impact factor: 3.490

3. A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12.

Authors: N B Vartak; L Liu; B M Wang; C M Berg
Journal: J Bacteriol Date: 1991-06 Impact factor: 3.490

Physiological characterization of polar Tn5-induced isoleucine-valine auxotrophs in Escherichia coli K.12: evidence for an internal promoter in the ilvOGEDA operon.

1. The effect of an ilvA Mu phage insertion on ilv gene expression in Escherichia coli K-12.

2. Acetylornithinase of Escherichia coli: partial purification and some properties.

3. Transduction of linked genetic characters of the host by bacteriophage P1.

4. The ilvEDA operon of Escherichia coli K12 encodes only one valine-alpha-ketoglutarate transaminase activity.

5. Regulation of isoleucine-valine biosynthesis in an ilvDAC deletion strain of Escherichia coli K-12.

6. Regulation of transaminase C synthesis in Escherichia coli: conditional leucine auxotrophy.

7. Polarity and the regulation of the ilv gene cluster in Escherichia coli strain K-12.

8. Multivalent regulation of isoleucine-valine transaminase in an Escherichia coli K-12 ilvA deletion strain.

9. Transaminase B from Escherichia coli: quaternary structure, amino-terminal sequence, substrate specificity, and absence of a separate valine-alpha-ketoglutarate activity.

10. Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5.

1. Further evidence that transposition of Tn5 in Escherichia coli is strongly enhanced by constitutively activated RecA proteins.

2. Multiple transcripts encoded by the ilvGMEDA gene cluster of Escherichia coli K-12.

3. A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12.

4. Tn5-Induced Mutations in the Enterobacterial Phytopathogen Erwinia chrysanthemi.

Review 5. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

6. Cloning and characterization of the Escherichia coli K-12 alanine-valine transaminase (avtA) gene.

7. Translational coupling between the ilvD and ilvA genes of Escherichia coli.

8. Analysis of an avtA::Mu d1(Ap lac) mutant: metabolic role of transaminase C.

9. Altered regulation of isoleucine-valine biosynthesis in a hisW mutant of Salmonella typhimurium.

10. Gratuitous repression of avtA in Escherichia coli and Salmonella typhimurium.