Literature DB >> 3949938

Determination of vitamin K in serum using HPLC with post-column reaction and fluorescence detection.

W E Lambert, A P De Leenheer, M F Lefevere.   

Abstract

A new, sensitive and selective detection method for vitamin K1 in human serum is reported. After the chromatographic separation, vitamin K is converted to its hydroquinone form in a wet-chemical post-column reduction reaction with tetramethylammonium octahydridotriborate. The reaction proceeds in an open tubular knitted reaction coil at elevated temperature. This system, when combined with a two-step multidimensional liquid chromatographic separation, has proven useful for the quantitative determination of serum concentrations of vitamin K1 in normal, healthy individuals.

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Year:  1986        PMID： 3949938     DOI： 10.1093/chromsci/24.2.76

Source DB:  PubMed          Journal:  J Chromatogr Sci        ISSN： 0021-9665            Impact factor:   1.618


  2 in total

1.  Effects of oral and intramuscular vitamin K prophylaxis on vitamin K1, PIVKA-II, and clotting factors in breast fed infants.

Authors:  E A Cornelissen; L A Kollée; R A De Abreu; J M van Baal; K Motohara; B Verbruggen; L A Monnens
Journal:  Arch Dis Child       Date:  1992-10       Impact factor: 3.791

2.  Plasma concentrations of vitamin K1 and PIVKA-II in bottle-fed and breast-fed infants with and without vitamin K prophylaxis at birth.

Authors:  J Widdershoven; W Lambert; K Motohara; L Monnens; A de Leenheer; I Matsuda; F Endo
Journal:  Eur J Pediatr       Date:  1988-11       Impact factor: 3.183
  2 in total

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