| Literature DB >> 3947642 |
Abstract
A simple method is described for the determination of both the pseudo-first-order rate constant and the second-order rate constant for enzyme inactivation by a chemical reagent which itself undergoes exponential decay. The validity of this method has been demonstrated in two test cases in which the labile diethyl pyrocarbonate was used to inactivate salicylate hydroxylase and bacterial luciferase.Entities:
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Year: 1986 PMID： 3947642 DOI： 10.1016/0167-4838(86)90076-2
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002