Literature DB >> 3947361

Purification and properties of the guanine nucleotide exchange factor (GEF) from HeLa cells and its role in the initiation of protein synthesis.

T M Mariano, J Siekierka, S Ochoa.   

Abstract

A guanine nucleotide exchange factor (GEF), catalyzing the exchange of GDP bound to initiation factor eIF-2 for GTP, has been isolated from S3 HeLa cells as the eIF-2 X GEF complex and extensively purified by procedures originally developed for purification of GEF from rabbit reticulocytes. The HeLa cell factor resembles rabbit reticulocyte eIF-2 X GEF in polypeptide composition, catalytic activity, and inactivation by alpha-phosphorylated eIF-2.

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Year:  1986        PMID： 3947361     DOI： 10.1016/0006-291x(86)90372-4

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  2 in total

1.  Effect of vasopressin on the regulation of protein synthesis initiation in liver cells.

Authors:  J Menaya; R Parrilla; M S Ayuso
Journal:  Biochem J       Date:  1988-09-15       Impact factor: 3.857

2.  Cloning and expression of cDNAs for the beta subunit of eukaryotic initiation factor-2B, the guanine nucleotide exchange factor for eukaryotic initiation factor-2.

Authors:  B L Craddock; N T Price; C G Proud
Journal:  Biochem J       Date:  1995-08-01       Impact factor: 3.857
  2 in total

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