Literature DB >> 3944470

A fluorescence NK assay using flow cytometry.

K McGinnes, G Chapman, R Marks, R Penny.   

Abstract

A flow cytometric NK assay was developed in which the K562 targets were labelled with the fluorogenic substrate, carboxyfluorescein diacetate (c'FDA). This new assay compared favourably with results obtained using the conventional 51Cr-release assay. c'FDA was not toxic to target cells and did not inhibit lysis. The assay permits the evaluation of various aspects of NK activity such as the activity of NK-enriched, IFN-alpha-activated, and ALG-inhibited populations. The assay can be used in place of 51Cr-release, and has the advantages of being able to directly monitor target cell lysis of reducing overall assay time, and the avoidance of radioisotope usage.

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Year:  1986        PMID： 3944470     DOI： 10.1016/0022-1759(86)90258-9

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  15 in total

1.  NK cells control breast cancer and related cancer stem cell hematological spread.

Authors:  Rossana Tallerico; Laura Conti; Stefania Lanzardo; Rosa Sottile; Cinzia Garofalo; Arnika K Wagner; Maria H Johansson; Costanza Maria Cristiani; Klas Kärre; Ennio Carbone; Federica Cavallo
Journal:  Oncoimmunology       Date:  2017-02-23       Impact factor: 8.110

2.  Purification and characterization of cytolytic and noncytolytic human natural killer cell subsets.

Authors:  L T Lebow; B Bonavida
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

3.  Application of an improved flow cytometry-based NK cell activity assay in adult hemophagocytic lymphohistiocytosis.

Authors:  Jia Zhang; Yini Wang; Lin Wu; Jingshi Wang; Ran Tang; Shuo Li; Jianhang Chen; Zhuo Gao; Ruijun Pei; Zhao Wang
Journal:  Int J Hematol       Date:  2017-02-09       Impact factor: 2.490

4.  Natural killer cell function in ankylosing spondylitis.

Authors:  D Wendling; E Racadot; M Guidet
Journal:  Ann Rheum Dis       Date:  1988-09       Impact factor: 19.103

5.  Natural killer cell activity in Sjögren's syndrome and systemic lupus erythematosus: stimulation with interferons and interleukin-2 and correlation with immune complexes.

Authors:  N J Struyf; H W Snoeck; C H Bridts; L S De Clerck; W J Stevens
Journal:  Ann Rheum Dis       Date:  1990-09       Impact factor: 19.103

6.  New pathogenetic hypothesis for Wolman disease: possible role of oxidized low-density lipoproteins in adrenal necrosis and calcification.

Authors:  G Fitoussi; A Nègre-Salvayre; M T Pieraggi; R Salvayre
Journal:  Biochem J       Date:  1994-07-01       Impact factor: 3.857

7.  Cytotoxicity of unstimulated and thrombin-activated platelets to human tumour cells.

Authors:  T Sagawa; A Tominaga; T Kodama; M Okada
Journal:  Immunology       Date:  1993-04       Impact factor: 7.397

8.  Chemosensitivity testing of human gliomas using a fluorescent microcarrier technique.

Authors:  A P Bowles; C G Pantazis; W Wansley; M B Allen
Journal:  J Neurooncol       Date:  1990-04       Impact factor: 4.130

9.  Protection by Ca2+ channel blockers (nifedipine, diltiazem and verapamil) against the toxicity of oxidized low density lipoprotein to cultured lymphoid cells.

Authors:  A Nègre-Salvayre; R Salvayre
Journal:  Br J Pharmacol       Date:  1992-11       Impact factor: 8.739

10.  Early hematopoietic zinc finger protein prevents tumor cell recognition by natural killer cells.

Authors:  Rosanna La Rocca; Mariateresa Fulciniti; Tadepally Lakshmikanth; Maria Mesuraca; Talib Hassan Ali; Valerio Mazzei; Nicola Amodio; Lucio Catalano; Bruno Rotoli; Ouathek Ouerfelli; Michele Grieco; Elio Gulletta; Heather M Bond; Giovanni Morrone; Soldano Ferrone; Ennio Carbone
Journal:  J Immunol       Date:  2009-04-15       Impact factor: 5.422
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