Heterogeneity of neurons in the crustacean X-organ as revealed by intracellular recording and injection of horseradish peroxidase.

The intracellular injection technique of HRP with simultaneous recordings of intracellular potential was applied to the crab X-organ-sinus gland peptidergic neurosecretory neurons. At least two classes of neurons were discriminated from the usual type of neurosecretory neurons morphologically as well as electrophysiologically. Possible roles of those neurons were suggested as the modulation and coordination of activities of the neurosecretory system.