Literature DB >> 3937594

An evaluation of various plastic embedding methods for preparing ground sections from calcified tissue with the Logitech system for light microscopy.

F T Wong.   

Abstract

Three plastic processing methods for preparing ground sections from hard tissue applicable to light microscopy are reviewed. The requirement for production of high quality ground sections of tooth and bone for pathological diagnosis is discussed and a new machine system technology, namely the LP 30 Lapping Machine (Logitech Ltd., Dunbartonshire, Scotland) which meets these requirements is described. The new approach is justified by the results, since sections of quality are produced with increased efficiency, while the operator's working conditions are distinctly improved. Ground sections, 10-20 microns, are being produced from glycol methacrylate impregnated specimens with the employment of the Leitz diamond saw and the Logitech section grounding system. Good GMA ground sections form an invaluable aid to microscopic diagnosis of many disorders of enamel as well as metabolic bone diseases.

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Year:  1985        PMID: 3937594     DOI: 10.1007/bf02554928

Source DB:  PubMed          Journal:  Calcif Tissue Int        ISSN: 0171-967X            Impact factor:   4.333


  7 in total

1.  Water-soluble methacrylate as an embedding medium for the preparation of ultrathin sections.

Authors:  M ROSENBERG; P BARTL; J LESKO
Journal:  J Ultrastruct Res       Date:  1960-12

2.  A simplified method for the preparation of methyl methacrylate embedding medium for undecalcified bone.

Authors:  J Difford
Journal:  Med Lab Technol       Date:  1974-01

3.  an improved histological method for undecalcified bone sections.

Authors:  J C Johnstone; C S Tam
Journal:  Med Lab Technol       Date:  1973-10

4.  A review of three resin processing techniques applicable to light microscopy.

Authors:  R B Janes
Journal:  Med Lab Sci       Date:  1979-07

5.  Hydroxyethyl methacrylate combined with polyethylene glycol 400 and water; and embedding medium for routine 1-2 micron sectioning.

Authors:  C L Ruddell
Journal:  Stain Technol       Date:  1967-05

6.  Effects of acid-dissolution on thin ground sections of enamel caries studied by microradiography and x-ray microbeam diffraction.

Authors:  T Aoba; T Yagi
Journal:  J Oral Pathol       Date:  1982-06

7.  Glycol methacrylate in light microscopy: a routine method for embedding and sectioning animal tissues.

Authors:  M B Cole; S M Sykes
Journal:  Stain Technol       Date:  1974-11
  7 in total

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