Literature DB >> 3937368

A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human red blood cells.

Y Katsumata, M Sato.   

Abstract

A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human erythrocytes is described. Specific antibodies were first absorbed with the corresponding cells, then eluted by heating at 53 degrees-55 degrees C for 15 min. The method is simple and highly efficient with a fair recovery of 15.6%-34.4%.

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Year:  1985        PMID: 3937368     DOI: 10.1007/bf00201074

Source DB:  PubMed          Journal:  Z Rechtsmed        ISSN: 0044-3433


  4 in total

1.  Affinity-purified Ulex europaeus lectin: preparation and serological characteristics.

Authors:  P W Harris-Smith; S M Fletcher
Journal:  J Forensic Sci Soc       Date:  1983-07

2.  [Immunoadsorbents for the purification of MN blood group system antibodies].

Authors:  R Iida; K Ago; O Tsuganezawa
Journal:  Nihon Hoigaku Zasshi       Date:  1983-04

3.  [Studies on the purification of anti-A allohemagglutinin by affinity chromatography. (1) Comparative efficiency of several eluting methods].

Authors:  K Ago; O Tsuganezawa
Journal:  Nihon Hoigaku Zasshi       Date:  1984-02

4.  Studies on human antibodies. IV. Purification and properties of anti-A and anti-B obtained by absorption and elution from insoluble blood group substances.

Authors:  M E Kaplan; E A Kabat
Journal:  J Exp Med       Date:  1966-06-01       Impact factor: 14.307

  4 in total

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