Literature DB >> 3936495

Stoichiometry of binding of high molecular weight kininogen to factor XI/XIa.

B J Warn-Cramer, S P Bajaj.   

Abstract

Factor XI is a dimeric protein and circulates in plasma complexed with high molecular weight kininogen (HMWK). We investigated the binding of HMWK to factor XIa utilizing two active site directed fluorescent probes: nitrobenzoxadiazole aminopentyl methylphosphonofluoridate for serine and dansyl-glu-gly-arg-chloromethyl ketone for histidine. In the presence of saturating amounts of HMWK, the fluorescence of factor XIa-fluorophore was quenched by approximately 28% for each probe. Titrations of the fluorescent factor XIa with HMWK revealed that each subunit of factor XIa binds one molecule of HMWK with a Kd approximately 3.4 X 10(-8)M.

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Year:  1985        PMID： 3936495     DOI： 10.1016/0006-291x(85)90922-2

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

Review 1.  Bradykinin formation. Plasma and tissue pathways and cellular interactions.

Authors:  A P Kaplan; K Joseph; Y Shibayama; Y Nakazawa; B Ghebrehiwet; S Reddigari; M Silverberg
Journal:  Clin Rev Allergy Immunol       Date:  1998       Impact factor: 8.667

2.  Antibody-probed conformational transitions in the protease domain of human factor IX upon calcium binding and zymogen activation: putative high-affinity Ca(2+)-binding site in the protease domain.

Authors:  S P Bajaj; A K Sabharwal; J Gorka; J J Birktoft
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

3.  Intrinsic versus extrinsic coagulation. Kinetic considerations.

Authors:  B J Warn-Cramer; S P Bajaj
Journal:  Biochem J       Date:  1986-11-01       Impact factor: 3.857

4.  Mechanism of activation of coagulation factor XI by factor XIIa studied with monoclonal antibodies.

Authors:  H Akiyama; D Sinha; F S Seaman; E P Kirby; P N Walsh
Journal:  J Clin Invest       Date:  1986-12       Impact factor: 14.808

5.  Inhibitor of the factor VIIa-tissue factor complex is reduced in patients with disseminated intravascular coagulation but not in patients with severe hepatocellular disease.

Authors:  M S Bajaj; S V Rana; R B Wysolmerski; S P Bajaj
Journal:  J Clin Invest       Date:  1987-06       Impact factor: 14.808

6.  First epidermal growth factor-like domain of human blood coagulation factor IX is required for its activation by factor VIIa/tissue factor but not by factor XIa.

Authors:  D Zhong; K J Smith; J J Birktoft; S P Bajaj
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-26       Impact factor: 11.205

7.  Characterization of Novel Forms of Coagulation Factor XIa: independence of factor XIa subunits in factor IX activation.

Authors:  Stephen B Smith; Ingrid M Verhamme; Mao-fu Sun; Paul E Bock; David Gailani
Journal:  J Biol Chem       Date:  2008-01-11       Impact factor: 5.157
  7 in total

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