| Literature DB >> 3936344 |
Abstract
Diamine oxidase or its substrates can be measured using a coupled reaction with peroxidase and the chromogen 3-methyl-2-benzothiazolone hydrazone (MBTH) with an appropriate acceptor such as 3-(dimethylamino)benzoic acid (DMAB). This method provides a more rapid and sensitive method of measuring histamine than current colorimetric assays; using this system, histamine was quantitated at concentrations of 10 to 400 mumol/l. The Km of DAO was determined to be 2.9 X 10(-5) M, a lower value than previously reported.Entities:
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Year: 1985 PMID： 3936344 DOI： 10.1007/bf01966672
Source DB: PubMed Journal: Agents Actions ISSN： 0065-4299