Interaction of dietary tryptophan and social isolation on territorial aggression, motor activity, and neurochemistry in mice.

This study examined the interaction of dietary tryptophan (TRP) and differential housing on territorial-induced aggression, locomotor activity, and monoamine neurochemistry in mice. Groups of male CF-1 mice were singly-housed or group-housed and administered a semisynthetic basal diet supplemented with TRP (0.25-1.0%). Behavioral measures were taken at various intervals up to 2 weeks after dietary administration was instituted. Separate groups of mice were given the same experimental treatment and sacrificed for whole brain determination of the monoamines and their metabolites. Isolated mice were consistently more aggressive than grouped animals, suggesting that territorial-induced aggression is synergistic with intermale aggression based on social isolation. The combination of isolation and 0.50% TRP was particularly effective in producing increases in aggression that reached maximal levels after 10 days of diet administration. However, motor activity of singly-housed mice was unaffected by TRP, while that of grouped mice was decreased after 5 days of 0.50% TRP. By day 14 of administration behavioral changes tended to return to baseline levels. Neurochemical measures indicated increased DA and 5-HT turnover in isolated mice, with the 5-HT system most affected by dietary TRP. Because housing conditions were a prominent factor in the aggression and neurochemistry, the results suggest the involvement of both transmitter systems in this behavior. However, there were no changes in monoamine turnover that could account for the development of behavioral tolerance.