Methodological aspects of the evaluation of the binding of drugs to plasma and tissue proteins.

This paper describes the methodological aspects of the evaluation of the binding of drugs to plasma and tissue proteins. The relevance of the procedures employed to certain problems in clinical-pharmacology as well as the role of plasma binding in drug distribution and pharmacokinetics are discussed. The possibly important role of plasma protein binding of some drugs is emphasized. The incidence of plasma binding on drug distribution and pharmacokinetics are developed and the possible incidence of pathological states on drug plasma binding are considered.