| Literature DB >> 3934683 |
P L Bruynzeel, P T Kok, R J Viëtor, J Verhagen.
Abstract
The optimal conditions for the -in vitro- LTC4 formation by the human eosinophil, isolated from peripheral blood, have been investigated in detail. LTC4 formation was found strongly Ca2+ and ionophore dependent and was complete after 20 min. Maximal LTC4 production was observed in the presence of 2 mM Ca2+, 10 microM ionophore A23187 and 5 mM glutathione. Addition of arachidonic acid resulted in a significant inhibition of the LTC4-synthesis by human eosinophils. In contrast, the formation of 15-HETE was strongly stimulated by the addition of arachidonic acid. As the LTC4 synthesis was found to be strongly inhibited by the addition of 15(S)-HETE to the incubation medium, this monohydroxy acid may be responsible for the inhibitory activity of arachidonic acid.Entities:
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Year: 1985 PMID： 3934683 DOI： 10.1016/0262-1746(85)90090-3
Source DB: PubMed Journal: Prostaglandins Leukot Med ISSN： 0262-1746