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Literature DB >> 3934207

Modified taurocholate-tellurite-gelatin agar for improved differentiation of Vibrio species.

Abstract

A total of 78 strains, representing 21 Vibrio species, were examined by using taurocholate-tellurite-gelatin agar (TTGA) medium and modified TTGA medium containing 4-methylumbelliferyl-beta-D-galactoside (150 micrograms/ml). Modified TTGA medium allowed for simple and direct detection of beta-D-galactosidase (beta-gal) activity. This feature, in conjunction with other differential characteristics of TTGA medium, gave improved differentiation of the vibrios tested. The modified TTGA medium allowed for easy differentiation of Vibrio cholerae (beta-gal+) from Vibrio parahaemolyticus (beta-gal-). The 4-methylumbelliferyl-beta-D-galactoside substrate is inexpensive and very stable. Incorporation into the agar did not affect the performance of TTGA as a differential medium. The assay for beta-gal activity with this substrate was specific and sensitive.

Entities: Chemical Species

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Year: 1985 PMID： 3934207 PMCID： PMC271868 DOI： 10.1128/jcm.22.6.1011-1013.1985

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

9 in total

1. Identification of Pseudomonas pyocyanea by the oxidase reaction.

Authors: N KOVACS
Journal: Nature Date: 1956-09-29 Impact factor: 49.962

2. A highly selective gelatin-taurocholate-tellurite medium for the isolation of Vibrio cholerae.

Authors: K A MONSUR
Journal: Trans R Soc Trop Med Hyg Date: 1961-09 Impact factor: 2.184

3. The rapid detection of lactose fermentation in paracolon organisms by the demonstration of beta-D-galactosidase.

Authors: G H LOWE
Journal: J Med Lab Technol Date: 1962-01

4. Evaluation of thiosulfate-citrate-bile salts-sucrose agar, a selective medium for the isolation of Vibrio cholerae and other pathogenic vibrios.

Authors: W M McCormack; W E DeWitt; P E Bailey; G K Morris; P Soeharjono; E J Gangarosa
Journal: J Infect Dis Date: 1974-05 Impact factor: 5.226

5. Laboratory methods in cholera: isolation of Vibrio cholerae (el tor and classical) on TCBS medium in minimally equipped laboratories.

Authors: E J Gangarosa; W E Dewitt; I Huq; A Zarifi
Journal: Trans R Soc Trop Med Hyg Date: 1968 Impact factor: 2.184

6. Methylumbelliferyl-beta-D-glucuronide-based medium for rapid isolation and identification of Escherichia coli.

Authors: R W Trepeta; S C Edberg
Journal: J Clin Microbiol Date: 1984-02 Impact factor: 5.948

7. Comparison of four plating media for isolating Vibrio cholerae.

Authors: G K Morris; M H Merson; I Huq; A K Kibrya; R Black
Journal: J Clin Microbiol Date: 1979-01 Impact factor: 5.948

8. Enzymatic profile of Pseudomonas maltophilia.

Authors: M O'Brien; G H Davis
Journal: J Clin Microbiol Date: 1982-09 Impact factor: 5.948

9. Semiquantitative catalase test as an aid in identification of oxidative and nonsaccharolytic gram-negative bacteria.

Authors: B Chester
Journal: J Clin Microbiol Date: 1979-10 Impact factor: 5.948

9 in total

4 in total

4. In Situ Analyses Directly in Diarrheal Stool Reveal Large Variations in Bacterial Load and Active Toxin Expression of Enterotoxigenic Escherichiacoli and Vibrio cholerae.

Authors: Yasmin Ara Begum; Hanna A Rydberg; Kaisa Thorell; Young-Keun Kwak; Lei Sun; Enrique Joffré; Firdausi Qadri; Åsa Sjöling
Journal: mSphere Date: 2018-01-24 Impact factor: 4.389