Literature DB >> 3934183

Cell surface galactosyltransferase: immunochemical localization.

N L Shaper, P L Mann, J H Shaper.   

Abstract

A cell surface UDP-galactose:N-acetylglucosamine galactosyltransferase (GT) has been directly localized on bovine cells in tissue culture by immunohistochemical techniques. A conventional rabbit heteroantiserum was prepared against an affinity-purified soluble form of GT from bovine milk, and a monospecific IgG fraction was isolated by affinity chromatography on a GT adsorbent. As demonstrated by indirect immunofluorescence, antigen to this antibody is present on the surface of all three bovine cell lines tested. It was uniformly distributed over the exposed membrane surface of fixed cells. Exposure of living cells to the anti-GT antibody resulted in its time-dependent aggregation in the plane of the membrane. Antigen (GT) was released from the membrane surface by trypsin digestion, and its reappearance required protein synthesis, since cycloheximide effectively prevented repopulation of the cell surface.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 3934183     DOI： 10.1002/jcb.240280305

Source DB:  PubMed          Journal:  J Cell Biochem        ISSN： 0730-2312            Impact factor:   4.429


  14 in total

1.  Identification and characterization of glucose transport proteins in plasma membrane- and Golgi vesicle-enriched fractions prepared from lactating rat mammary gland.

Authors:  R J Madon; S Martin; A Davies; H A Fawcett; D J Flint; S A Baldwin
Journal:  Biochem J       Date:  1990-11-15       Impact factor: 3.857

2.  Unusual binding sites for horseradish peroxidase on the surface of cultured and isolated mammalian cells. Suppression of binding by certain nucleotides and glycoproteins, and a role for calcium.

Authors:  W Straus; J M Keller
Journal:  Histochemistry       Date:  1986

3.  DS28-6, a temperature-sensitive mutant of Chinese hamster ovary cells, expresses key phenotypic changes associated with brefeldin A treatment.

Authors:  C Zuber; J Roth; T Misteli; A Nakano; K Moremen
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-01       Impact factor: 11.205

4.  Competition between ligands of glycosyltransferases and horseradish peroxidase for binding sites on intracellular and plasma membranes of HeLa cells. Application of a micro-method for the semi-quantitation of surface-bound HRP.

Authors:  W Straus; J M Keller
Journal:  Histochemistry       Date:  1990

5.  Murine beta 1,4-galactosyltransferase: both the amounts and structure of the mRNA are regulated during spermatogenesis.

Authors:  N L Shaper; W W Wright; J H Shaper
Journal:  Proc Natl Acad Sci U S A       Date:  1990-01       Impact factor: 11.205

6.  Cholesterol-independent targeting of Golgi membrane proteins in insect cells.

Authors:  M M Rolls; M T Marquardt; M Kielian; C E Machamer
Journal:  Mol Biol Cell       Date:  1997-11       Impact factor: 4.138

7.  Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library.

Authors:  N L Shaper; J H Shaper; J L Meuth; J L Fox; H Chang; I R Kirsch; G F Hollis
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

8.  Binding sites for horseradish peroxidase on the cell surface. Suppression of binding by gangliosides and effects of some bivalent cations.

Authors:  W Straus; J M Keller
Journal:  Histochemistry       Date:  1987

9.  Laminin induces the stable expression of surface galactosyltransferase on lamellipodia of migrating cells.

Authors:  D J Eckstein; B D Shur
Journal:  J Cell Biol       Date:  1989-06       Impact factor: 10.539

10.  The response of the Golgi complex to microtubule alterations: the roles of metabolic energy and membrane traffic in Golgi complex organization.

Authors:  J R Turner; A M Tartakoff
Journal:  J Cell Biol       Date:  1989-11       Impact factor: 10.539
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.