Literature DB >> 3932337

Crystallization and preliminary x-ray investigation of purine-nucleoside phosphorylase from Escherichia coli.

W J Cook, S E Ealick, T A Krenitsky, J D Stoeckler, J R Helliwell, C E Bugg.   

Abstract

Crystals of purine-nucleoside phosphorylase from Escherichia coli have been grown from solutions of ammonium sulfate. The crystals are hexagonal with space group P6(1)22 or P6(5)22; the axes are alpha = 106.5 A and c = 241.3 A. The crystals are moderately stable to x-rays and diffract beyond 3.0-A resolution. It appears that the molecule, which is a hexamer, utilizes the 2-fold symmetry of the space group, resulting in three subunits/asymmetric unit.

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Year:  1985        PMID： 3932337

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  2 in total

1.  Investigation of alpha-deuterium kinetic isotope effects on the purine nucleoside phosphorylase reaction by the equilibrium-perturbation technique.

Authors:  P K Lehikoinen; M L Sinnott; T A Krenitsky
Journal:  Biochem J       Date:  1989-01-15       Impact factor: 3.857

2.  Acholeplasma laidlawii B-PG9 adenine-specific purine nucleoside phosphorylase that accepts ribose-1-phosphate, deoxyribose-1-phosphate, and xylose-1-phosphate.

Authors:  M C McElwain; M V Williams; J D Pollack
Journal:  J Bacteriol       Date:  1988-02       Impact factor: 3.490
  2 in total

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