Effect of indomethacin and hydrocortisone upon joint tissue in vitro. Incorporation of [35S]sulphate into chondrocyte proteoglycans.

It has previously been shown that products from the synovial tissue influence the behaviour of the chondrocyte in such a way that, not only is the degradation enhanced, but the synthesis of cartilage proteoglycans too is inhibited. In the case of cartilage degradation, these cellular interactions within the joint tissues have been shown to be partly modulated by arachidonic acid metabolites. The experiments reported here show that these cellular interactions cannot be completely ruled out in the case of cartilage synthesis either. Conditioned medium from synovial tissue (control SM) reduced the incorporation of [35S]sulphate into the monolayer cultures of chondrocytes. This reduction was not affected with the simultaneous addition of indomethacin (1.4 X 10(-6) mol/l) to the chondrocyte cultures. However, conditioned synovial medium from synovial tissue that had been cultured with indomethacin (1.4 X 10(-5) mol/l) enhanced radiosulphate incorporation, somewhat, as compared with control SM. Control SM together with hydrocortisone (2.2 X 10(-8) mol/l, 2.2 X 10(-7) mol/l, 2.2 X 10(-6) mol/l) reduced radiosulphate incorporation into the chondrocyte cultures in a dose-dependent manner. Conditioned synovial medium from synovial tissue that had been cultured with hydrocortisone (2.2 X 10(-8) mol/l, 2.2 X 10(-7) mol/l, 2.2 X 10(-6) mol/l) enhanced radiosulphate incorporation, as compared with control SM.