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Literature DB >> 3929245

Transcription of Drosophila small hsp-tk hybrid genes is induced by heat shock and by ecdysterone in transfected Drosophila cells.

Abstract

Hybrid genes containing the 5' region of Drosophila heat shock protein (hsp) genes, ligated to the herpesvirus thymidine kinase (tk) gene, were transfected into Drosophila line S3 cells. Constructions containing sequences upstream from hsp70, or from any of the small hsp genes, showed heat-inducible tk expression. Ecdysterone-inducible tk expression was seen only in transfections with small hsp-tk hybrid genes. Plasmids containing a synthetic "heat shock consensus sequence" or a deletion of the hsp23 upstream region were totally inactive in transfection studies.

Entities: Chemical Disease Gene Species

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Year: 1985 PMID： 3929245 PMCID： PMC390654 DOI： 10.1073/pnas.82.17.5865

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

35 in total

1. Transient expression of homologous genes in Drosophila cells.

Authors: C M Morganelli; E M Berger
Journal: Science Date: 1984-06-01 Impact factor: 47.728

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

3. Demonstration of an ecdysteroid receptor in a cultured cell line of Drosophila melanogaster.

Authors: P Maroy; R Dennis; C Beckers; B A Sage; J D O'Connor
Journal: Proc Natl Acad Sci U S A Date: 1978-12 Impact factor: 11.205

4. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors: P S Thomas
Journal: Proc Natl Acad Sci U S A Date: 1980-09 Impact factor: 11.205

5. Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies.

Authors: R Dudler; A A Travers
Journal: Cell Date: 1984-09 Impact factor: 41.582

6. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.

Authors: C Wu
Journal: Nature Date: 1980-08-28 Impact factor: 49.962

7. A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene.

Authors: C S Parker; J Topol
Journal: Cell Date: 1984-05 Impact factor: 41.582

8. Synthesis of low molecular weight heat shock peptides stimulated by ecdysterone in a cultured Drosophila cell line.

Authors: R C Ireland; E M Berger
Journal: Proc Natl Acad Sci U S A Date: 1982-02 Impact factor: 11.205

9. Expression of heat shock-beta-galactosidase hybrid genes in cultured Drosophila cells.

Authors: R Lawson; R Mestril; P Schiller; R Voellmy
Journal: Mol Gen Genet Date: 1984

10. Integration, transcription, and control of a Drosophila heat shock gene in mouse cells.

Authors: V Corces; A Pellicer; R Axel; M Meselson
Journal: Proc Natl Acad Sci U S A Date: 1981-11 Impact factor: 11.205

16 in total

1. Sequences involved in temperature and ecdysterone-induced transcription are located in separate regions of a Drosophila melanogaster heat shock gene.

Authors: E Hoffman; V Corces
Journal: Mol Cell Biol Date: 1986-02 Impact factor: 4.272

2. Two or more copies of Drosophila heat shock consensus sequence serve to activate transcription in yeast.

Authors: R Wei; H Wilkinson; K Pfeifer; C Schneider; R Young; L Guarente
Journal: Nucleic Acids Res Date: 1986-10-24 Impact factor: 16.971

3. The ovarian, ecdysterone, and heat-shock-responsive promoters of the Drosophila melanogaster hsp27 gene react very differently to perturbations of DNA sequence.

Authors: E P Hoffman; S L Gerring; V G Corces
Journal: Mol Cell Biol Date: 1987-03 Impact factor: 4.272

4. Sequence requirement for expression of the Drosophila melanogaster heat shock protein hsp22 gene during heat shock and normal development.

Authors: R Klemenz; W J Gehring
Journal: Mol Cell Biol Date: 1986-06 Impact factor: 4.272

5. A consensus sequence polymer inhibits in vivo expression of heat shock genes.

Authors: H Xiao; J T Lis
Journal: Mol Cell Biol Date: 1986-09 Impact factor: 4.272

6. Efficient transcription of a Caenorhabditis elegans heat shock gene pair in mouse fibroblasts is dependent on multiple promoter elements which can function bidirectionally.

Authors: R J Kay; R J Boissy; R H Russnak; E P Candido
Journal: Mol Cell Biol Date: 1986-09 Impact factor: 4.272