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Literature DB >> 3929232

Purification of antibodies for the cytokinin N6-(delta 2-isopentenyl) adenosine by affinity chromatography.

Abstract

Isopentenyl adenosine antibodies useful in the investigations of the "cytokinin" functions of isopentenyl adenosine were purified by affinity chromatography. Using different affinity columns, the antibodies were purified to near complete purity. Analyses of the purified proteins revealed the presence of isopentenyl adenosine binding proteins in normal rabbit serum, which presence supports a suggested role for isopentenyl adenosine and its related compounds in animal cell division in vivo.

Entities: Chemical Species

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Year: 1985 PMID： 3929232 PMCID： PMC321915 DOI： 10.1093/nar/13.16.5833

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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References

27 in total

1. A unique method utilizing antinucleotide antibodies for evaluating changes in the levels of modified nucleosides of tRNAs from crude extracts of whole cells.

Authors: B S Vold; H W Nolen
Journal: Nucleic Acids Res Date: 1979-10-25 Impact factor: 16.971

2. Ribosome structure: localization of 3' end of RNA in small subunit by immunoelectronmicroscopy.

Authors: H M Olson; D G Glitz
Journal: Proc Natl Acad Sci U S A Date: 1979-08 Impact factor: 11.205

3. The preparation of nucleotide-protein conjugates: carbodiimides as coupling agents.

Authors: M J Halloran; C W Parker
Journal: J Immunol Date: 1966-03 Impact factor: 5.422

4. N 6 -( 2 -Isopentenyl)adenosine: the regulatory effects of a cytokinin and modified nucleoside from tRNA on human lymphocytes.

Authors: R C Gallo; S M Hecht; J Whang-Peng; S O'Hopp
Journal: Biochim Biophys Acta Date: 1972-11-09

5. Toxicological and antiproliferative effects of N6-(delta2-isopentenyl) adenosine, a natural component of mammalian transfer RNA.

Authors: D Suk; C L Simpson; E Mihich
Journal: Cancer Res Date: 1970-05 Impact factor: 12.701

10. Antibodies to N6-(delta2-isopentenyl) adenosine and its nucleotide: interaction with purified tRNAs and with bases, nucleosides and nucleotides of the isopentenyladenosine family.

Authors: D S Milstone; B S Vold; D G Glitz; N Shutt
Journal: Nucleic Acids Res Date: 1978-09 Impact factor: 16.971

Purification of antibodies for the cytokinin N6-(delta 2-isopentenyl) adenosine by affinity chromatography.

1. A unique method utilizing antinucleotide antibodies for evaluating changes in the levels of modified nucleosides of tRNAs from crude extracts of whole cells.

2. Ribosome structure: localization of 3' end of RNA in small subunit by immunoelectronmicroscopy.

3. The preparation of nucleotide-protein conjugates: carbodiimides as coupling agents.

4. N 6 -( 2 -Isopentenyl)adenosine: the regulatory effects of a cytokinin and modified nucleoside from tRNA on human lymphocytes.

5. Toxicological and antiproliferative effects of N6-(delta2-isopentenyl) adenosine, a natural component of mammalian transfer RNA.

6. High resolution detection of DNA-RNA hybrids in situ by indirect immunofluorescence.

7. Evidence for an unusual immunological response to isopentenyladenosine in rabbits.

8. Localization of 5-methylcytosine in human metaphase chromosomes by immunoelectron microscopy.

9. Ribosome structure: localization of N6,N6-dimethyladenosine by electron microscopy of a ribosome-antibody complex.

10. Antibodies to N6-(delta2-isopentenyl) adenosine and its nucleotide: interaction with purified tRNAs and with bases, nucleosides and nucleotides of the isopentenyladenosine family.