Literature DB >> 3926869

A fluorescence staining method for the demonstration and measurement of lysosomal enzyme activities in single cells.

G P Luyten, A T Hoogeveen, H Galjaard.   

Abstract

A cytochemical fluorescence method is described that makes possible simple, rapid, and specific demonstration and measurement of the activities of a wide variety of lysosomal enzymes in single cells using 4-methylumbelliferyl derivatives as substrates. The validity of the method and a number of applications using normal and mutant human cells are presented.

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Year:  1985        PMID: 3926869     DOI: 10.1177/33.9.3926869

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN: 0022-1554            Impact factor:   2.479


  6 in total

Review 1.  Assessment of lysosomal function by quantitative histochemical and cytochemical methods.

Authors:  C J van Noorden
Journal:  Histochem J       Date:  1991-10

2.  The role of cytochemistry in human genetic research.

Authors:  H Galjaard
Journal:  Histochemistry       Date:  1986

3.  Histochemical detection of alpha-D-glucosidases and their molecular forms with 5-Br-4-Cl-3-indoxyl-alpha-D-glucoside.

Authors:  R Gossrau; Z Lojda; M Ruhnke
Journal:  Histochemistry       Date:  1987

4.  Irreversible loss of the oestrogen receptor in T47D breast cancer cells following prolonged oestrogen deprivation.

Authors:  J J Pink; M M Bilimoria; J Assikis; V C Jordan
Journal:  Br J Cancer       Date:  1996-10       Impact factor: 7.640

5.  Cloning and characterization of a 77-kDa oestrogen receptor isolated from a human breast cancer cell line.

Authors:  J J Pink; M Fritsch; M M Bilimoria; V J Assikis; V C Jordan
Journal:  Br J Cancer       Date:  1997       Impact factor: 7.640

6.  The oestrogen-like effect of 4-hydroxytamoxifen on induction of transforming growth factor alpha mRNA in MDA-MB-231 breast cancer cells stably expressing the oestrogen receptor.

Authors:  A S Levenson; D A Tonetti; V C Jordan
Journal:  Br J Cancer       Date:  1998-06       Impact factor: 7.640

  6 in total

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