| Literature DB >> 3926797 |
R Valencia, H N'Guyen Công, O Bertaux.
Abstract
Separation of the major bases of DNA along with seven minor methylated ones was obtained by reversed-phase high-performance liquid chromatography using an isocratic elution system. Application of this procedure to DNA treated in vitro by N-methyl-N-nitrosourea has allowed identification of two induced minor bases; a third one was resolved using a slightly modified mobile phase. Baseline resolution of 3-methyl- and 5-methylcytosine, detected in Euglena DNA hydrolyzates, was also achieved.Entities:
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Year: 1985 PMID： 3926797 DOI： 10.1016/s0021-9673(00)96021-7
Source DB: PubMed Journal: J Chromatogr