Literature DB >> 3925811

A simplified in situ solubilization procedure for the determination of DNA and cell number in tissue cultured mammalian cells.

D C West, A Sattar, S Kumar.   

Abstract

A rapid and simple procedure for the direct solubilization of DNA from adherent cultured cells, or sedimented cells, has been developed and employed in the measurement of DNA in small numbers of endothelial cells (1 X 10(3).

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Year:  1985        PMID： 3925811     DOI： 10.1016/0003-2697(85)90274-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  36 in total

1.  Detection of antiendothelial cell antibodies by an enzyme-linked immunosorbent assay using antigens from cell lysate: minimal interference with antinuclear antibodies and rheumatoid factors.

Authors:  Christian Drouet; Marie-France Nissou; Denise Ponard; Josiane Arvieux; Chantal Dumestre-Pérard; Philippe Gaudin; Bernard Imbert; Christian Massot; Françoise Sarrot-Reynauld
Journal:  Clin Diagn Lab Immunol       Date:  2003-09

2.  Evaluation of osteoblast-like cell response to Proroot MTA (mineral trioxide aggregate) cement.

Authors:  G A Pelliccioni; G Ciapetti; E Cenni; D Granchi; M Nanni; S Pagani; A Giunti
Journal:  J Mater Sci Mater Med       Date:  2004-02       Impact factor: 3.896

3.  Impaired elastogenesis in Hurler disease: dermatan sulfate accumulation linked to deficiency in elastin-binding protein and elastic fiber assembly.

Authors:  A Hinek; S E Wilson
Journal:  Am J Pathol       Date:  2000-03       Impact factor: 4.307

4.  UV absorption as an approximation for cell number in in vitro toxicity testing.

Authors:  M J Chang
Journal:  Bull Environ Contam Toxicol       Date:  1991-08       Impact factor: 2.151

5.  Characterization of aggregation and protein expression of bovine corneal endothelial cells as microcarrier cultures in a rotating-wall vessel.

Authors:  J W Muhitch; K C O'Connor; D A Blake; D J Lacks; N Rosenzweig; G F Spaulding
Journal:  Cytotechnology       Date:  2000-03       Impact factor: 2.058

6.  Comparative analysis of satellite cell properties in heavy- and lightweight strains of turkey.

Authors:  F Merly; C Magras-Resch; T Rouaud; J Fontaine-Perus; M F Gardahaut
Journal:  J Muscle Res Cell Motil       Date:  1998-04       Impact factor: 2.698

7.  Vibration stimulates vocal mucosa-like matrix expression by hydrogel-encapsulated fibroblasts.

Authors:  Jaishankar K Kutty; Ken Webb
Journal:  J Tissue Eng Regen Med       Date:  2010-01       Impact factor: 3.963

8.  A rapid and ultrasensitive method for measurement of DNA, calcium and protein content, and alkaline phosphatase activity of chondrocyte cultures.

Authors:  C C Teixeira; M Hatori; P S Leboy; M Pacifici; I M Shapiro
Journal:  Calcif Tissue Int       Date:  1995-03       Impact factor: 4.333

9.  Inhibition of type 5 adenovirus infectivity by periodate oxidation.

Authors:  G Ogier; Y Michal; V Thomas; G Quash; J D Rodwell
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

10.  Upregulation of fibronectin synthesis by interleukin-1 beta in coronary artery smooth muscle cells is associated with the development of the post-cardiac transplant arteriopathy in piglets.

Authors:  N Clausell; M Rabinovitch
Journal:  J Clin Invest       Date:  1993-10       Impact factor: 14.808
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