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Literature DB >> 3923486

Secretory S complex of Bacillus subtilis forms a large, organized structure when released from ribosomes.

M P Caulfield, D Furlong, P C Tai, B D Davis.

Abstract

The S complex of Bacillus subtilis, a set of four proteins that appears to be involved in protein secretion, is shown to be attached to 70S ribosomes: antibody to its 64-kDa component can aggregate these ribosomes, and the complex can be chemically crosslinked to ribosomal proteins. Low Mg2+ or prolonged high-speed centrifugation in a sucrose gradient releases the S complex from the ribosomes, and it is recovered as an aggregate with an S value of 76. Electron microscopy shows that these aggregates have a regular structure, somewhat resembling clathrin cages, with a diameter of about 45 nm. If these aggregates are physiological, their function would differ significantly from that of the signal recognition particle of eukaryotes.

Entities: Chemical Species

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Year: 1985 PMID： 3923486 PMCID： PMC397928 DOI： 10.1073/pnas.82.12.4031

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

22 in total

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3. Electron microscopy of an antibody-hapten complex.

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4. Pressure-induced dissociation of sedimenting ribosomes: effect on sedimentation patterns.

Authors: A A Infante; R Baierlein
Journal: Proc Natl Acad Sci U S A Date: 1971-08 Impact factor: 11.205

5. A 64-kilodalton membrane protein of Bacillus subtilis covered by secreting ribosomes.

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Journal: Proc Natl Acad Sci U S A Date: 1983-06 Impact factor: 11.205

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Authors: S Horiuchi; D Marty-Mazars; P C Tai; B D Davis
Journal: J Bacteriol Date: 1983-06 Impact factor: 3.490

7. The 64-kilodalton membrane protein of Bacillus subtilis is also present as a multiprotein complex on membrane-free ribosomes.

Authors: M P Caulfield; S Horiuchi; P C Tai; B D Davis
Journal: Proc Natl Acad Sci U S A Date: 1984-12 Impact factor: 11.205

8. Coated vesicles transport newly synthesized membrane glycoproteins from endoplasmic reticulum to plasma membrane in two successive stages.

Authors: J E Rothman; R E Fine
Journal: Proc Natl Acad Sci U S A Date: 1980-02 Impact factor: 11.205

9. Polymerization of clathrin protomers into basket structures.

Authors: P P Van Jaarsveld; P K Nandi; R E Lippoldt; H Saroff; H Edelhoch
Journal: Biochemistry Date: 1981-07-07 Impact factor: 3.162

10. In vitro translocation of bacterial proteins across the plasma membrane of Escherichia coli.

Authors: M Müller; G Blobel
Journal: Proc Natl Acad Sci U S A Date: 1984-12 Impact factor: 11.205

5 in total

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Authors: Q P Weng; L L Chen; P C Tai
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