Literature DB >> 3922781

Magnesium enhances human pancreatic elastase digestion of 125I-labeled elastin.

M Rabaud, F Lefebvre, P V Graves, C Desgranges, H Bricaud.   

Abstract

The effect of some divalent cations, especially Mg++, on elastinolysis by porcine or human pancreatic elastase has been determined using 125Iodine-labeled elastin as substrate. Elastin degradation was significantly increased in the presence of 10(-3) M Mg++. If elastin was pre-incubated with 0.5 (w/v) Triton, there was a further increase in elastinolysis to 2.6 times the original rate.

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Year:  1985        PMID: 3922781     DOI: 10.1007/bf02007692

Source DB:  PubMed          Journal:  Experientia        ISSN: 0014-4754


  14 in total

1.  Studies on elastase and elastin. I. Assay and properties of elastase.

Authors:  F LAMY; C P CRAIG; S TAUBER
Journal:  J Biol Chem       Date:  1961-01       Impact factor: 5.157

2.  Pancreatic elastase: purification, properties, and function.

Authors:  N G BRINK; U J LEWIS; D E WILLIAMS
Journal:  J Biol Chem       Date:  1956-10       Impact factor: 5.157

3.  Radioimmunoassay for pancreatic carboxypeptidase B in human serum.

Authors:  M C Geokas; F Wollesen; H Rinderknecht
Journal:  J Lab Clin Med       Date:  1974-10

4.  Characterization of collagen peptides by sodium dodecylsulfate-polyacrylamide electrophoresis.

Authors:  H Furthmayr; R Timpl
Journal:  Anal Biochem       Date:  1971-06       Impact factor: 3.365

5.  Determination of elastolytic activity with 125-I and 131-I labelled elastin.

Authors:  B Robert; L Robert
Journal:  Eur J Biochem       Date:  1969-11

6.  A new radioactive assay for enzymes with elastolytic activity using reduced tritiated elastin. The effect of sodium dodecyl sulfate on elastolysis.

Authors:  S Takahashi; S Seifter; F C Yang
Journal:  Biochim Biophys Acta       Date:  1973-11-15

7.  Proteolysis of elastin-ligand complexes. Stimulation of elastase digestion of insoluble elastin by sodium dodecyl sulfate.

Authors:  H M Kagan; G D Crombie; R E Jordan; W Lewis; C Franzblau
Journal:  Biochemistry       Date:  1972-08-29       Impact factor: 3.162

8.  Changes in the state of ionization of carboxyl groups in elastin in response to the binding of sodium dodecyl sulfate.

Authors:  H M Kagan
Journal:  Connect Tissue Res       Date:  1978       Impact factor: 3.417

9.  Purification and characterization of two human pancreatic elastases.

Authors:  C Largman; J W Brodrick; M C Geokas
Journal:  Biochemistry       Date:  1976-06-01       Impact factor: 3.162

10.  Effect of Mg2+ and Ca2+ on enzymatic elastolysis of insoluble elastin determined by a conductimetric method.

Authors:  F Bernier; H Bakala; J Wallach
Journal:  Connect Tissue Res       Date:  1981       Impact factor: 3.417

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