Literature DB >> 3922355

Measurement of xylanase activity with insoluble xylan substrate.

M Nummi, J M Perrin, M L Niku-Paavola, T M Enari.   

Abstract

Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.

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Year:  1985        PMID： 3922355      PMCID： PMC1144752          DOI： 10.1042/bj2260617

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  3 in total

Review 1.  WOOD HEMICELLULOSES. I.

Authors:  T E TIMELL
Journal:  Adv Carbohydr Chem       Date:  1964

2.  Nephelometric and turbidometric assays of cellulase activity.

Authors:  M Nummi; P C Fox; M L Niku-Paavola; T M Enari
Journal:  Anal Biochem       Date:  1981-09-01       Impact factor: 3.365

3.  Cellobiohydrolase from Trichoderma reesei.

Authors:  M Nummi; M L Niku-Paavola; A Lappalainen; T M Enari; V Raunio
Journal:  Biochem J       Date:  1983-12-01       Impact factor: 3.857
  3 in total
  5 in total

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Authors:  P Wang; P Broda
Journal:  Appl Environ Microbiol       Date:  1992-10       Impact factor: 4.792

2.  Biochemical and kinetic characterization of GH43 β-D-xylosidase/α-L-arabinofuranosidase and GH30 α-L-arabinofuranosidase/β-D -xylosidase from rumen metagenome.

Authors:  Jungang Zhou; Lei Bao; Lei Chang; Yufei Zhou; Hong Lu
Journal:  J Ind Microbiol Biotechnol       Date:  2011-07-02       Impact factor: 3.346

3.  A new appraisal of the endoglucanases of the fungus Trichoderma reesei.

Authors:  M L Niku-Paavola; A Lappalainen; T M Enari; M Nummi
Journal:  Biochem J       Date:  1985-10-01       Impact factor: 3.857

4.  Construction of recombinant sestc Saccharomyces cerevisiae for consolidated bioprocessing, cellulase characterization, and ethanol production by in situ fermentation.

Authors:  Peizhou Yang; Haifeng Zhang; Shaotong Jiang
Journal:  3 Biotech       Date:  2016-09-03       Impact factor: 2.406

5.  Expression, Docking, and Molecular Dynamics of Endo-β-1,4-xylanase I Gene of Trichoderma virens in Pichia stipitis.

Authors:  Gammadde Hewa Ishan Maduka Wickramasinghe; Pilimathalawe Panditharathna Attanayake Mudiyanselage Samith Indika Rathnayake; Naduviladath Vishvanath Chandrasekharan; Mahindagoda Siril Samantha Weerasinghe; Ravindra Lakshman Chundananda Wijesundera; Wijepurage Sandhya Sulochana Wijesundera
Journal:  Biomed Res Int       Date:  2017-08-10       Impact factor: 3.411
  5 in total

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