Literature DB >> 3920012

Induction of chromosome banding by trypsin/EDTA for gene mapping by in situ hybridization.

N C Popescu, S C Amsbaugh, D C Swan, J A DiPaolo.   

Abstract

We describe an easy and reproducible procedure that utilizes trypsin/EDTA for the induction of chromosome banding in conjunction with in situ hybridization. The high quality banding resolution required for grain localization is obtained on both elongated and contracted chromosomes derived from synchronized or nonsynchronized human lymphocytes or fibroblasts. This procedure can also be useful for gene localization on chromosomes from cancer cells.

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Year:  1985        PMID： 3920012     DOI： 10.1159/000132107

Source DB:  PubMed          Journal:  Cytogenet Cell Genet        ISSN： 0301-0171


  20 in total

1.  Mapping the human gene coding for chromosomal protein HMG-17.

Authors:  N Popescu; D Landsman; M Bustin
Journal:  Hum Genet       Date:  1990-08       Impact factor: 4.132

2.  Chromosomal protein HMG-14 gene maps to the Down syndrome region of human chromosome 21 and is overexpressed in mouse trisomy 16.

Authors:  J Pash; N Popescu; M Matocha; S Rapoport; M Bustin
Journal:  Proc Natl Acad Sci U S A       Date:  1990-05       Impact factor: 11.205

3.  Molecular characterization of a Y;15 translocation segregating in a family.

Authors:  T Alitalo; J Tiihonen; P Hakola; A de la Chapelle
Journal:  Hum Genet       Date:  1988-05       Impact factor: 4.132

4.  Characterization of a (Y;4) translocation by DNA hybridization.

Authors:  M Andersson; D C Page; L G Brown; K Elfving; A de la Chapelle
Journal:  Hum Genet       Date:  1988-04       Impact factor: 4.132

5.  The gene encoding human low-molecular weight insulin-like growth-factor binding protein (IGF-BP25): regional localization to 7p12-p13 and description of a DNA polymorphism.

Authors:  T Alitalo; K Kontula; R Koistinen; K Aalto-Setälä; M Julkunen; O A Jänne; M Seppälä; A de la Chapelle
Journal:  Hum Genet       Date:  1989-11       Impact factor: 4.132

6.  Localisation of satellite DNA sequences on human metaphase chromosomes using bromodeoxyuridine-labelled probes.

Authors:  M Frommer; C Paul; P C Vincent
Journal:  Chromosoma       Date:  1988       Impact factor: 4.316

7.  Characterization of a porcine glucosephosphate isomerase-processed pseudogene at chromosome 1q1.6-1.7.

Authors:  I Harbitz; B P Chowdhary; S Kran; W Davies
Journal:  Mamm Genome       Date:  1993       Impact factor: 2.957

8.  A pseudoautosomal minisatellite in the pig.

Authors:  E N Signer; F Gu; I Gustavsson; L Andersson; A J Jeffreys
Journal:  Mamm Genome       Date:  1994-01       Impact factor: 2.957

9.  The gene for the alpha polypeptide of pyruvate dehydrogenase is X-linked in humans.

Authors:  P Szabo; K F Sheu; R M Robinson; K H Grzeschik; J P Blass
Journal:  Am J Hum Genet       Date:  1990-05       Impact factor: 11.025

10.  Y;autosome translocations and mosaicism in the aetiology of 45,X maleness: assignment of fertility factor to distal Yq11.

Authors:  M Andersson; D C Page; D Pettay; I Subrt; C Turleau; J de Grouchy; A de la Chapelle
Journal:  Hum Genet       Date:  1988-05       Impact factor: 4.132
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