Literature DB >> 3919065

Enhanced luminescence enzyme immunoassay for factor VIII related antigen.

H X Wang, J George, G H Thorpe, R A Stott, L J Kricka, T P Whitehead.   

Abstract

A sandwich enzyme immunoassay for plasma factor VIII related antigen has been developed which exploits a para-iodophenol enhanced chemiluminescent reaction to detect the horseradish peroxidase label. The assay entailed 15 min incubations with sample and with conjugate and had a detection limit of 0.12 mU. It showed good within batch precision (coefficient of variation = 2.95-5.8%) and results on a series of 57 specimens agreed with results obtained by immunoelectrophoresis (correlation coefficient = 0.97).

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Year:  1985        PMID： 3919065      PMCID： PMC499133          DOI： 10.1136/jcp.38.3.317

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  9 in total

1.  Competitive enzyme-linked immunoassay for Factor VIII antigen.

Authors:  L D Yorde; C V Hussey; D E Yorde; E A Sasse
Journal:  Clin Chem       Date:  1979-11       Impact factor: 8.327

2.  The use of an immunoradiometric assay for factor VIII related antigen in the study of atypical Von Willebrand's disease.

Authors:  I R Peake; A L Bloom
Journal:  Thromb Res       Date:  1977-01       Impact factor: 3.944

3.  The role of phospholipid and factor VIIIa in the activation of bovine factor X.

Authors:  G van Dieijen; G Tans; J Rosing; H C Hemker
Journal:  J Biol Chem       Date:  1981-04-10       Impact factor: 5.157

4.  Factor VIII related antigen: an improved enzyme immunoassay.

Authors:  P E Short; C E Williams; A M Picken; F G Hill
Journal:  Med Lab Sci       Date:  1982-10

5.  Serial changes in coagulation and viscosity during sickle-cell crisis.

Authors:  S G Richardson; K B Matthews; J Stuart; A M Geddes; R M Wilcox
Journal:  Br J Haematol       Date:  1979-01       Impact factor: 6.998

6.  Factor-VIII-related antigen: measurement by enzyme immunoassay.

Authors:  A Bartlett; K M Dormandy; C M Hawkey; P Stableforth; A Voller
Journal:  Br Med J       Date:  1976-04-24

7.  Investigation of enzyme immunoassay time courses: development of rapid assay systems.

Authors:  R H Yolken; F J Leister
Journal:  J Clin Microbiol       Date:  1981-04       Impact factor: 5.948

8.  Detection of the carrier state for classic hemophilia using an enzyme-linked immunosorbent assay (ELISA).

Authors:  D J Fishman; P K Jones; J E Menitove; O D Ratnoff; B Everson
Journal:  Blood       Date:  1982-06       Impact factor: 22.113

9.  Detection of carriers of classic hemophilia using an immunologic assay for antihemophilic factor (factor 8().

Authors:  T S Zimmerman; O D Ratnoff; A S Littell
Journal:  J Clin Invest       Date:  1971-01       Impact factor: 14.808
  9 in total
  2 in total

1.  A luminescence Western blot with enhanced sensitivity for antibodies to human immunodeficiency virus.

Authors:  R Schneppenheim; P Rautenberg
Journal:  Eur J Clin Microbiol       Date:  1987-02       Impact factor: 3.267

2.  Quantitation of canine plasma von Willebrand factor antigen using a commercial enzyme-linked immunosorbent assay.

Authors:  I B Johnstone; S Crane
Journal:  Can J Vet Res       Date:  1991-01       Impact factor: 1.310
  2 in total

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