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Literature DB >> 391776

Fluorescent colloidal gold: a cytochemical marker for fluorescent and electron microscopy.

Abstract

The gold method was further developed for fluorescent microscopy. Gold granules (12 nm in size) were labelled with rhodamine conjugates of Concanavalin A and avidin. The fluorescent markers were used to mark cell wall mannan on the yeast Saccharomyces cerevisiae either by the one-step, or by the two-step method via a biotinyl derivative of ConA. By fluorescence or transmission electron microscopy, the two-step method was found to achieve a higher density of marking.

Entities: Chemical Species

Mesh：

Substances：

Year: 1979 PMID： 391776 DOI： 10.1007/bf00493360

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

12 in total

1. Preparation of ferritin-avidin conjugates by reductive alkylation for use in electron microscopic cytochemistry.

Authors: E A Bayer; E Skutelsky; D Wynne; M Wilchek
Journal: J Histochem Cytochem Date: 1976-08 Impact factor: 2.479

2. An immunocolloid method for the electron microscope.

Authors: W P Faulk; G M Taylor
Journal: Immunochemistry Date: 1971-11

3. A low-viscosity epoxy resin embedding medium for electron microscopy.

Authors: A R Spurr
Journal: J Ultrastruct Res Date: 1969-01

4. Lymphocyte membrane lectins. Direct visualization by the use of fluoresceinyl-glycosylated cytochemical markers.

Authors: C Kieda; A C Roche; F Delmotte; M Monsigny
Journal: FEBS Lett Date: 1979-03-15 Impact factor: 4.124

5. Coloidal gold, ferritin and peroxidase as markers for electron microscopic double labeling lectin techniques.

Authors: J Roth; M Binder
Journal: J Histochem Cytochem Date: 1978-03 Impact factor: 2.479

6. Simultaneous localization of an hepatic binding protein specific for galactose and of galactose-containing receptors on rat hepatocytes.

Authors: M Horisberger; M VonLanthen
Journal: J Histochem Cytochem Date: 1978-11 Impact factor: 2.479

Fluorescent colloidal gold: a cytochemical marker for fluorescent and electron microscopy.

1. Preparation of ferritin-avidin conjugates by reductive alkylation for use in electron microscopic cytochemistry.

2. An immunocolloid method for the electron microscope.

3. A low-viscosity epoxy resin embedding medium for electron microscopy.

4. Lymphocyte membrane lectins. Direct visualization by the use of fluoresceinyl-glycosylated cytochemical markers.

5. Coloidal gold, ferritin and peroxidase as markers for electron microscopic double labeling lectin techniques.

6. Simultaneous localization of an hepatic binding protein specific for galactose and of galactose-containing receptors on rat hepatocytes.

7. Use of the avidin-biotin complex for specific staining of biological membranes in electron microscopy.

8. Location of mannan and chitin on thin sections of budding yeasts with gold markers.

9. Conjugation of lectins with fluorochromes: an approach to histochemical double labeling of carbohydrate components.

10. New immunolatex spheres: visual markers of antigens on lymphocytes for scanning electron microscopy.

1. Immunophotoelectron microscopy: the electron optical analog of immunofluorescence microscopy.

2. Ultrastructural histochemistry in biomedical research: Alive and kicking.