Literature DB >> 3917281

The murine plasma cell antigen PC-1: purification and partial amino acid sequence.

P A Stearne, I R van Driel, B Grego, R J Simpson, J W Goding.   

Abstract

The murine plasma cell alloantigen PC-1 is selectively expressed on B lymphocytes in their terminal phase of differentiation into antibody-secreting cells. Previous work on an analytical scale has shown that PC-1 consists of two apparently identical disulfide-bonded polypeptides, each of Mr 115,000. In this paper, we describe the generation of a monoclonal antibody to PC-1 and its use in the preparative isolation of PC-1 by affinity chromatography. Final purification to apparent homogeneity was achieved by preparative polyacrylamide gel electrophoresis. It was estimated that NS-1 myeloma cells possess 1 to 4 X 10(5) PC-1 monomers per cell on their surface. The yield of PC-1 after purification was approximately 10(5) monomers per cell. Purified PC-1 was digested with trypsin, and the resulting peptides were separated by reversed-phase high-performance liquid chromatography. Purified peptides were sequenced with a gas-phase sequencer.

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Year:  1985        PMID： 3917281

Source DB:  PubMed          Journal:  J Immunol        ISSN： 0022-1767            Impact factor:   5.422


  8 in total

1.  Processing of rotavirus glycoprotein VP7: implications for the retention of the protein in the endoplasmic reticulum.

Authors:  S C Stirzaker; P L Whitfeld; D L Christie; A R Bellamy; G W Both
Journal:  J Cell Biol       Date:  1987-12       Impact factor: 10.539

2.  Style proteins of a wild tomato (Lycopersicon peruvianum) associated with expression of self-incompatibility.

Authors:  S L Mau; E G Williams; A Atkinson; M A Anderson; E C Cornish; B Grego; R J Simpson; A Kheyr-Pour; A E Clarke
Journal:  Planta       Date:  1986-10       Impact factor: 4.116

3.  Purification and characterization of two high-density-lipoprotein-binding proteins from rat and human liver.

Authors:  M Tozuka; N Fidge
Journal:  Biochem J       Date:  1989-07-01       Impact factor: 3.857

4.  Genetic nomenclature for loci controlling mouse lymphocyte antigens.

Authors:  H C Morse; F W Shen; U Hämmerling
Journal:  Immunogenetics       Date:  1987       Impact factor: 2.846

5.  Murine plasma cell membrane antigen PC-1: molecular cloning of cDNA and analysis of expression.

Authors:  I R van Driel; A F Wilks; G A Pietersz; J W Goding
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

6.  Characterization of monoclonal antibodies to the plasma cell alloantigen ENPP1.

Authors:  Sadia Abbasi; Dong-Mi Shin; Natalie Beaty; Marek Masiuk; Sophia Chen; Ines Gonzalez-Garcia; Ming Zhao; James Goding; Herbert C Morse; Hongsheng Wang
Journal:  Hybridoma (Larchmt)       Date:  2011-02

7.  Purification, renaturation, and reconstituted protein kinase activity of the Sendai virus large (L) protein: L protein phosphorylates the NP and P proteins in vitro.

Authors:  H Einberger; R Mertz; P H Hofschneider; W J Neubert
Journal:  J Virol       Date:  1990-09       Impact factor: 5.103

8.  Divalent cations stabilize the conformation of plasma cell membrane glycoprotein PC-1 (alkaline phosphodiesterase I).

Authors:  S I Belli; A Sali; J W Goding
Journal:  Biochem J       Date:  1994-11-15       Impact factor: 3.857
  8 in total

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